Inhibitory effects of homoharringtonine on foot and mouth disease virus in vitro
Inhibitory effects of homoharringtonine on foot and mouth disease virus in vitro
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高三尖杉酯碱对口蹄疫病毒的体外抑制作用
DOI：
10.1002/jmv.25494
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发表时间：
2019-09-01
影响因子：
12.7
通讯作者：
Chang, Hui-yun
中科院分区：
文献类型：
--
作者：
Gong, Mei-jiao;Li, Shi-fang;Chang, Hui-yun
口蹄疫（FMD）是一种高度传染性疾病，影响偶蹄动物，包括牛，猪，绵羊，山羊和许多野生物种。需要采取有效的控制措施。在此，我们表明高三尖杉酯碱处理在猪肾（IBRS-2）细胞中对两种不同的FMDV毒株（0/MYA 98/BY/2010和A/GD/MM/2013）表现出强抑制作用。进一步的实验表明，高三尖杉酯碱不影响病毒的附着或进入。使用添加时间测定，我们发现高三尖杉酯碱的抗病毒活性主要发生在感染的早期阶段。这些结果表明，高三尖杉酯碱可能是一种有效的抗口蹄疫病毒药物。高三尖杉酯碱在体内抗口蹄疫病毒复制的活性有待进一步研究。
Foot-and-mouth disease (FMD) is a highly contagious disease that affects cloven-hoof animals including cattle, swine, sheep, goats, and lots of wild species. Effectively control measures are urged needed. Here, we showed that homoharringtonine treatment exhibited a strong inhibitory effect against two different strains of FMDVs (O/MYA98/BY/2010 and A/GD/MM/2013) in swine kidney (IBRS-2) cells. Further experiments demonstrated that homoharringtonine did not affect virus attachment or entry. Using time-of-addition assays, we found that the antiviral activity of homoharringtonine occurred primarily during the early stage of infection. These results demonstrated that homoharringtonine might be an effective anti-FMDV drug. Further studies are required to explore the antiviral activity of homoharringtonine against FMDV replication in vivo.