Increased inflammatory response of blood cells to repeated bout of endurance exercise
Increased inflammatory response of blood cells to repeated bout of endurance exercise
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DOI：
10.1249/01.mss.0000227315.93351.8d
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发表时间：
2006-07-01
期刊：
影响因子：
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通讯作者：
Osterud, Bjarne
中科院分区：
文献类型：
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作者：
Degerstrom, Jorid;Osterud, Bjarne
目的：本研究的目的是评估两个30分钟的连续运动回合在跑步机上80%（V）超过barO（2 max），间隔4小时休息时间，对血细胞计数和生产的组织因子，细胞因子，类花生酸脂多糖（LPS）刺激的血液。研究方法：在每次运动前和运动后立即采集8名耐力运动员的血样（平均值± SD：年龄，23.4 ± 1.6岁;（V）超过barO（2 max），66.0 ± 6.4 L.min（-1）.kg（-1））。进行细胞计数，肝素化血液接受LPS刺激2 h。结果如下：第一次运动后白色细胞计数显著升高（81%，P < 0.001），第二次运动后增加到123%（P < 0.001）。在第一次和第二次运行后，与基线值相比，LPS刺激的单核细胞中的组织因子活性分别增强了70%（不显著）和几乎200%（P = 0.012）。在第二回合后的高单核细胞反应性在212天后仍然没有减弱。类似地，相对于基线值，第一次运行后白细胞介素（IL）-8的产生增加了70%（P = 0.022），第二次运行后增加了100%（P = 0.005）。IL-6或白三烯B-4水平无显著变化。LPS诱导的血栓素B-2在第一次运行后升高80%（P = 0.024），在第二次运行后升高63%（P = 0.071，不显著）。(V)第二次运动后即刻的粒细胞浓度与0. 864（P = 0. 006）呈负相关。结论：这项研究的结果是证据，两个体育锻炼回合分开4小时的休息与增强倾向的血细胞产生组织因子的活动和一些促炎产物相比，一个运动回合。
Purpose: The aim of the present study was to assess the effects of two 30-min consecutive exercise bouts on a treadmill at 80% (V) over barO(2max) separated by a 4-h rest interval, on blood cell counts and the production of tissue factor, cytokines, and eicosanoids in lipopolysaccharide (LPS)-stimulated blood. Methods: Blood samples were taken from eight endurance athletes (mean +/- SD: age, 23.4 +/- 1.6 yr; (V) over barO(2max), 66.0 +/- 6.4 L.min(-1).kg(-1)), both immediately before and after each exercise bout. Cell counts were performed, and the heparinized blood was subjected to LPS-stimulation for 2 h. Results: There was a significant rise in white blood cell counts after the first exercise bout (81%, P < 0.001), increasing to 123% (P < 0.001) after the second bout. After the first and second runs, the tissue factor activity in LPS-stimulated monocytes was enhanced by 70% (not significant) and almost 200% (P = 0.012), respectively, compared with baseline values. The high monocyte responsiveness after the second bout remained undiminished 2 It later. Similarly, the interleukin (IL)-8 production had risen 70% (P = 0.022) after the first run and 100% (P = 0.005) after the second run, relative to baseline values. IL-6 or leukotriene B-4 levels did not change significantly. The rise in LPS-induced thromboxane B-2 was 80% (P = 0.024) after the first run and 63% after the second run (P = 0.071, not significant). (V) over barO(2max) correlated negatively with the concentration of granulocytes immediately after the second exercise bout (R = 0.864, P = 0.006). Conclusions: The results of this study are evidence that two physical exercise bouts separated by a 4-h rest are associated with an enhanced propensity of the blood cells to generate tissue factor activity and some proinflammatory products compared with one exercise bout.