Intrakinetochore localization and essential functional domains of Drosophila Spc105
Intrakinetochore localization and essential functional domains of Drosophila Spc105
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DOI：
10.1038/emboj.2009.188
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发表时间：
2009-08-19
期刊：
影响因子：
11.4
通讯作者：
Lehner, Christian F.
中科院分区：
文献类型：
--
作者：
Schittenhelm, Ralf B.;Chaleckis, Romanas;Lehner, Christian F.
着丝粒在染色体的着丝粒区有丝分裂和减数分裂时组装。它由80多种不同的蛋白质组成。Spc105（在不同的真核生物中也被称为Spc7、KNL-1或Blinkin）是一种较大的着丝点蛋白，可以结合Mis12/MIND和Ndc80复合体以及纺锤体组装检查点成分Bub1和BubR1。我们对果蝇Spc105的遗传特征分析表明，缺少快速进化的、重复的中心三分之一的截断版本仍然提供所有基本功能。此外，与之前观察到的从内部延伸到外部着丝点区域的Cenp-C相比，全长Spc105的位置更远，并且没有类似地沿着纺锤轴延伸。因此,我们的研究结果表明,Spc105形式无论是扩展链接连接内部Cenp-A染色质与着丝粒区域外,也不是脚手架制约着丝粒复形和纺锤体组装检查站在一起成一个几何刚性超级复杂的组件。Spc105似乎在外部着丝点内提供了一个平台，允许各种着丝点组分的独立组装。EMBO杂志，2009,28,2374-2386。doi: 10.1038 / emboj.2009.188;2009年7月9日在线发布
The kinetochore is assembled during mitotic and meiotic divisions within the centromeric region of chromosomes. It is composed of more than eighty different proteins. Spc105 (also designated as Spc7, KNL-1 or Blinkin in different eukaryotes) is a comparatively large kinetochore protein, which can bind to the Mis12/MIND and Ndc80 complexes and to the spindle assembly checkpoint components Bub1 and BubR1. Our genetic characterization of Drosophila Spc105 shows that a truncated version lacking the rapidly evolving, repetitive central third still provides all essential functions. Moreover, in comparison with Cenp-C that has previously been observed to extend from the inner to the outer kinetochore region, full-length Spc105 is positioned further out and is not similarly extended along the spindle axis. Thus, our results indicate that Spc105 forms neither an extended link connecting inner Cenp-A chromatin with outer kinetochore regions nor a scaffold constraining kinetochore subcomplexes and spindle assembly checkpoint components together into a geometrically rigid super-complex. Spc105 seems to provide a platform within the outer kinetochore allowing independent assembly of various kinetochore components. The EMBO Journal (2009) 28, 2374-2386. doi:10.1038/emboj.2009.188;Published online 9 July 2009