Salvianolic acid A positively regulates PTEN protein level and inhibits growth of A549 lung cancer cells
Salvianolic acid A positively regulates PTEN protein level and inhibits growth of A549 lung cancer cells
复制标题
丹酚酸A正向调节PTEN蛋白水平并抑制A549肺癌细胞的生长
DOI：
10.3892/br.2012.33
复制
发表时间：
2013-03-01
影响因子：
2.3
通讯作者：
Chen, Weiping
中科院分区：
文献类型：
--
作者：
Bi, Lei;Chen, Jianping;Chen, Weiping
丹参酸A（Salvioleticacid A，Sal A）是从丹参中提取的一种有效成分，用于治疗多种疾病。初步数据表明，Sal A治疗具有特异性抗肺癌作用。然而，Sal A调节癌症生长的方式仍然未知。在这项研究中，A549肺癌细胞系及其对Sal A处理的反应进行了检查。结果表明，Sal A处理显著抑制A549细胞生长，促进部分凋亡，增加线粒体膜通透性。Western印迹分析表明，Sal A上调磷酸酶和张力蛋白同源物（PTEN）蛋白水平，同时一致下调Akt磷酸化。这些结果表明，Sal A负介导A549肺癌细胞系的生长或凋亡，最有可能通过正调节PTEN蛋白水平。
Salvianolic acid A (Sal A) is an effective compound extracted from Salvia miltiorrhiza which has been used in the treatment of various diseases. Preliminary data indicate that Sal A treatment has a specific anti-lung cancer effect. However, the manner in which Sal A regulates cancer growth remains unknown. In this study, the A549 lung cancer cell line and its response to Sal A treatment was examined. Results showed that Sal A treatment significantly decreased A549 cell growth, promoted partial apoptosis and increased mitochondrial membrane permeability. Western blot analysis showed that Sal A upregulated the phosphatase and tensin homolog (PTEN) protein level, while consistently downregulating Akt phosphorylation. These results indicate that Sal A negatively mediates A549 lung cancer cell line growth or apoptosis, most likely by positively regulating PTEN protein level.