Assay systems for screening food components that have anti-proliferative and anti-invasive activity to rat ascites hepatoma cells: In vitro and ex vivo effects of green tea extract
Assay systems for screening food components that have anti-proliferative and anti-invasive activity to rat ascites hepatoma cells: In vitro and ex vivo effects of green tea extract
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DOI：
10.1023/a:1007951231617
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发表时间：
1997-01-01
期刊：
影响因子：
2.2
通讯作者：
Yagasaki, K
中科院分区：
文献类型：
--
作者：
Miura, Y;Shiomi, H;Yagasaki, K
我们开发了体外和离体测定系统，用于筛选抑制大鼠腹水肝癌细胞系 AH109A 增殖和/或侵袭的食品成分和天然物质，并用它们来研究绿茶提取物的作用。在 10% 新生牛血清存在下，发现 AH109A 细胞渗透到从 Donryu 大鼠肠系膜分离的原代培养间皮细胞的单层下方。绿茶提取物以剂量依赖性方式抑制 AH109A 渗透，并且还以剂量依赖性方式抑制 AH109A 体外增殖。绿茶单宁是绿茶提取物中的主要多酚物质，在体外也以剂量依赖性方式抑制AH109A的增殖和侵袭。当经口插管0.5小时获得的大鼠血清以10%的浓度代替新生牛血清添加到培养基中时，与对照大鼠血清（绿茶提取物插管前）相比，AH109A的侵袭被显着抑制；口服绿茶提取物后，抑制作用持续1小时，3小时后消失，但大鼠血清未显示对AH109A增殖的抑制作用。这些结果表明，绿茶提取物在体外和离体均对AH109A的侵袭具有抑制作用，但仅在体外对AH109A的增殖具有抑制作用，并且这些测定系统可有效筛选抑制肿瘤细胞增殖和侵袭的食物成分。
We have developed in vitro and ex vivo assay systems for screening food components and natural substances that suppress the proliferation and/or invasion of a rat ascites hepatoma cell line of AH109A and have used them to study the effect of green tea extract. AH109A cells were found to penetrate underneath the monolayer of primary cultured mesothelial cells isolated from Donryu rat mesentery in the presence of 10% newborn bovine serum. Green tea extract inhibited this AH109A penetration in a dose dependent manner and also inhibited AH109A proliferation in vitro dose-dependently. Green tea tannin, the major polyphenolic substances in green tea extract, also inhibited the proliferation and invasion of AH109A in vitro in a dose dependent manner. When rat serum obtained 0.5 h after oral intubation of green tea extract was added to the culture media instead of newborn bovine serum at a concentration of 10%, the invasion of AH109A was significantly inhibited as compared to control rat serum (before green tea extract intubation); the inhibitory effect lasted for 1 h and disappeared 3 h after oral intubation of green tea extract, but those rat sera showed no inhibition of AH109A proliferation. These results suggest that green tea extract has an inhibitory effect on the invasion of AH109A both in vitro and ex vivo, but on the proliferation of AH109A only in vitro, and that these assay systems are effective for the screening of food components which inhibit tumor cell proliferation and invasion.