Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA.
Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA.
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快速标记 HeLa 细胞核 RNA。
DOI：
10.1016/s0022-2836(66)80009-8
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发表时间：
1966
影响因子：
5.6
通讯作者：
J. Darnell
中科院分区：
文献类型：
--
作者：
Jonathan R. Warner;Ruy Soeiro;H. Birnboim;Marc Girard;J. Darnell
对HeLa细胞RNA的快速标记作了进一步研究。前面描述的45 s核糖体前体RNA可以在区带沉降实验中从异质的20至80 s快速标记的种类中分离。在4 ~ 5分钟内掺入RNA的[3 H]尿苷中，有50%存在于这种异质组分中。当细胞用放线菌素处理时，大多数快速标记的RNA都来自这类RNA。
The rapidly labeled RNA from HeLa cells has been further studied. The previously described 45 s ribosomal precursor RNA can be separated in zone sedimentation experiments from a heterogeneous 20 to 80 s rapidly labeled species. As much as 50% of the [3H]uridine incorporated into RNA in four to five minutes is found in this heterogeneous fraction. The majority of the rapidly labeled RNA which decays when cells are treated with actinomycin comes from this RNA class.