Morphology, toxicity, and phylogeny of Alexandrium (Dinophyceae) species along the coast of China
Morphology, toxicity, and phylogeny of Alexandrium (Dinophyceae) species along the coast of China
复制标题
中国沿海亚历山大藻（甲藻纲）物种的形态、毒性和系统发育
DOI：
10.1016/j.hal.2013.05.008
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发表时间：
2013-07-01
期刊：
影响因子：
6.6
通讯作者：
Krock, Bernd
中科院分区：
文献类型：
--
作者：
Gu, Haifeng;Zeng, Ni;Krock, Bernd
产毒属Alexandrium包括近30种，但在区域尺度上关于其生物地理的信息有限。在这项研究中，我们通过孵化从7个地点收集的休眠囊来探索中国沿海亚历山大菌的多样性。共发现亚历山大菌231株，隶属于7个形态种。其中，andersonii Alexandrium、fraterculum Alexandrium、leei Alexandrium、pseudogonyaulax Alexandrium和tamutum Alexandrium均为首次在中国海域记录。部分大亚基（LSU）和（或）内部转录间隔区（ITS1、ITS2和5.8S rDNA）序列显示andersonii Alexandrium leei和tamarense Alexandrium的2种核型：Atama complex Group I和IV. Atama complex Group I仅分布于黄海和渤海，而Group IV仅分布于东中国海和南海。Atama复合物I组主要产生n -磺甲氨基酰基毒素（C1/C2，占总毒素的61-79%）和gonyautoxins （GTX1/4, 17-37%）。亚历山大菌ASBH01只产生NEO和STX（分别为65%和35%）。我们的研究结果支持了Atama复合体I群在亚太地区特有的前提，包括冷水物种，而Atama复合体IV群倾向于栖息在温暖的水域。(C) 2013 Elsevier B.V.版权所有
The toxigenic genus Alexandrium includes similar to 30 species, but information about its biogeography at a regional scale is limited. In this study, we explored the diversity of Alexandrium along the coast of China by incubating resting cysts collected from 7 sites. A total of 231 strains of Alexandrium belonging to 7 morphospecies were found. Among them, Alexandrium andersonii, Alexandrium fraterculum, Alexandrium leei, Alexandrium pseudogonyaulax, and Alexandrium tamutum were recorded from the China Sea for the first time. Partial large subunit (LSU) and/or internal transcribed spacer region (ITS1, ITS2, and 5.8S rDNA) sequences revealed two ribotypes of Alexandrium andersonii, Alexandrium leei, and Alexandrium tamarense: Atama complex Group I and IV. Atama complex Group I was exclusively distributed in the Yellow Sea and the Bohai Sea, whereas Group IV was restricted to the East China Sea and South China Sea. Atama complex Group I produced mainly N-sulfocarbamoyl toxins (C1/C2, 61-79% of total toxins) and gonyautoxins (GTX1/4, 17-37%). Alexandrium ostenfeldii strain ASBH01 produced NEO and STX exclusively (65% and 35%, respectively). Our results support the premise that Atama complex Group I is endemic to the Asian Pacific and includes cold water species, whereas Atama complex Group IV tends to inhabit warmer waters. (C) 2013 Elsevier B.V. All rights reserved.