Lignin Extraction and Quantification, a Tool to Monitor Defense Reaction at the Plant Cell Wall Level
Lignin Extraction and Quantification, a Tool to Monitor Defense Reaction at the Plant Cell Wall Level
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木质素提取和定量，一种监测植物细胞壁水平防御反应的工具
DOI：
10.21769/bioprotoc.1430
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A. Schikora
中科院分区：
文献类型：
--
作者：
Sebastian T. Schenk;A. Schikora
[摘要]木质素是酚类化合物（单木质素）的复杂聚合物，其有助于植物细胞壁的刚性。木质化是植物生长发育所必需的，同时也是植物防御机制之一。木质素的积累和单木质素在病原体攻击侧的聚合保护细胞壁免受细胞壁降解酶的侵害，从而防止病原体的渗透。除了酚类化合物的交联之外，这种抗性机制还包括胼胝质和纤维素在细胞壁上的沉积。这导致称为乳突的结构，其提供对真菌附着胞施加的机械压力的必要阻力。因此，木质素在细胞壁中的积累是植物防御反应的一部分。在这里，我们描述了一种定量方法的木质素和细胞壁酚类化合物，这是基于酸催化反应，导致在一个有色的和可溶的木质素-巯基乙酸盐复合物适合光度测量。
[Abstract] Lignin is a complex polymer of phenolic compounds (monolignins), which contributes to the rigidity of the plant cell wall. Lignification is essential for plant development, however it is also one of the mechanisms of plant defense. Accumulation of lignin and the polymerization of monolignins at sides of pathogen attack protect the cell wall against cell wall-degrading enzymes and prevent therefore the pathogen’s penetration. In addition to cross-linkage of phenolic compounds, this resistance mechanism includes also callose and cellulose appositions on the cell wall. This results in structures called papillae, which provide the necessary resistance to the mechanical pressure exercised by fungal appressorium. Lignin accumulation in cell walls is therefore a part of plant defense responses. Here we describe a quantification method for lignin and cell wall phenolic compounds, which is based on an acid-catalyzed reaction resulting in a colored and soluble lignin-thioglycolate complex suitable for photometric measurements.
影响因子：
3.2
作者：
Eynck, C.;Koopmann, B.;von Tiedemann, A.
通讯作者：
von Tiedemann, A.