A new extraction method for Acinetobacter species ODB-L2 rough form lipopolysaccharide from culture broth.
A new extraction method for Acinetobacter species ODB-L2 rough form lipopolysaccharide from culture broth.
复制标题
从培养液中提取不动杆菌ODB-L2粗型脂多糖的新方法。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
S. Fukuoka
中科院分区：
文献类型：
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作者：
K. Kunii;S. Nakamura;C. Sato;S. Fukuoka
革兰氏阴性细菌不动杆菌属物种ODB - L2在培养液中产生脂多糖（LPS）。使用90%苯酚/水或90%苯酚/氯仿/石油醚混合溶剂的常规提取方法无法纯化该脂多糖。采用氯仿、乙醇和4M盐酸溶液的混合物进行提取得以实现。通过将粗提取物溶解在90%苯酚中，并加入甲醇形成脂多糖沉淀来纯化脂多糖。通过化学、生化和物理化学方法对脂多糖进行表征，其为富含3 - 羟基十二烷酸的粗糙型脂多糖。
The Gram-negative bacterium Acinetobacter species ODB-L2 produces lipopolysaccharide (LPS) in culture broth. The LPS could not be purified by conventional extraction methods using 90% phenol/water or 90% phenol/chloroform/petroleum ether mixed solvent. Extraction was achieved employing an admixture of chloroform, ethanol, and 4 M HCI solution. The LPS was purified from dissolving the crude extracts in 90% phenol and LPS sediment formed by addition of methanol. The LPS was characterized by chemical, biochemical, and physicochemical methods as rough form 3-hydroxydodecanoic acid rich LPS.