Surface marker for hemopoietic and endothelial cell lineages in quail that is defined by a monoclonal antibody.
Surface marker for hemopoietic and endothelial cell lineages in quail that is defined by a monoclonal antibody.
复制标题
由单克隆抗体定义的鹌鹑造血细胞和内皮细胞谱系的表面标记。
DOI：
--
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
N. L. Le Douarin
中科院分区：
文献类型：
--
作者：
B. Péault;J. Thiery;N. L. Le Douarin
一种针对鹌鹑免疫球蛋白μ链的小鼠单克隆抗体被发现对鹌鹑（Coturnix coturnix japonica）的造血细胞和内皮细胞表现出广泛的反应性。在胚胎发育的几个阶段，直到孵化后3周，对分离的细胞或组织切片进行间接免疫荧光测定。他们揭示了定义的表面标志物，称为MB 1，（i）在胚胎内和胚胎外造血干细胞上早期表达，并传递给这些前体细胞的整个后代，成熟红细胞除外，（ii）是整个个体发育和成年生活中内皮细胞表面的恒定特征。此外，该表位包含在几种可溶性血浆组分中。MB 1在鸡组织中未检测到表达，这一特征被用来证实其在鹌鹑-鸡嵌合体中的谱系限制。我们强调的价值，这种物种和谱系特异性标志物的造血和内皮细胞家族的发展研究，特别提到他们可能的早期共同胚胎起源。
A mouse monoclonal antibody raised in response to quail immunoglobulin mu chain was found to exhibit a broad reactivity towards hemopoietic and endothelial cells in the quail (Coturnix coturnix japonica). Indirect immunofluorescence assays were performed at several stages of embryonic development and until 3 weeks after hatching, on either isolated cells or tissue sections. They revealed that the defined surface marker, referred to as MB1, (i) is expressed early on both intra- and extraembryonic hemopoietic stem cells and is transmitted to the whole progeny of these precursors, with the exception of mature erythrocytes, and (ii) is a constant feature of the endothelial cell surface throughout ontogenesis and adult life. In addition, this epitope is included in several soluble plasma components. MB1 expression was not detected in chicken tissues, and this characteristic was used to confirm its lineage restriction in quail-chicken chimeras. We stress the value of this species- and lineage-specific marker in study of the development of the hemopoietic and endothelial cell families, with special reference to their possible early common embryonic origin.