Detection of adulterated murine components in meat products by TaqMan© real-time PCR
Detection of adulterated murine components in meat products by TaqMan© real-time PCR
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DOI：
10.1016/j.foodchem.2015.07.020
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发表时间：
2016-02-01
期刊：
影响因子：
8.8
通讯作者：
Zhang, Chi
中科院分区：
文献类型：
--
作者：
Fang, Xin;Zhang, Chi
用鼠肉代羊肉在国内已被认定为一种新型肉类欺诈行为，但尚未见鼠类检测方法的报道。在此，以三种啮齿动物为目标物种，建立了小鼠特异性实时PCR检测方法。对每个靶标的线粒体细胞色素b基因(cytb)进行测序，并基于cytb设计TaqMan探针。同时，设计了内部阳性对照 (IPC) 质粒及其各自的探针来监测 PCR 反应。结果，验证了双重实时PCR系统的特异性。每次反应的检测限 (LOD) 低于 1 pg DNA，肉类混合物中的鼠类污染为 0.1%。生成标准曲线用于定量分析。因此，这项研究为官方和第三方实验室提供了控制肉制品质量的新工具。 (C) 2015 年，爱思唯尔有限公司出版。
Using murine meat to substitute mutton has been identified as a new type of meat fraud in China, yet no detection method for murine species has been reported. Here, three kinds of rodent were used as target species to establish a murine-specific real-time PCR method of detection. The mitochondrial cytochrome b gene (cytb) of each target was sequenced and a TaqMan probe was designed based on the cytb. Simultaneously, an internal positive control (IPC) plasmid along with its respective probe were designed to monitor the PCR reaction. As a result, the duplex real-time PCR system was verified to be specific. The limit of detection (LOD) was lower than 1 pg of DNA per reaction and 0.1% murine contamination in meat mixtures. Standard curves were generated for a quantitative analysis. Thus, this study provided a new tool to control the quality of meat products for official and third-party laboratories. (C) 2015 Published by Elsevier Ltd.