Chemical modification of amino acids by atmospheric-pressure cold plasma in aqueous solution
Chemical modification of amino acids by atmospheric-pressure cold plasma in aqueous solution
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DOI：
10.1088/0022-3727/47/28/285403
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发表时间：
2014-07-16
影响因子：
3.4
通讯作者：
Kitano, Katsuhisa
中科院分区：
文献类型：
--
作者：
Takai, Eisuke;Kitamura, Tsuyoshi;Kitano, Katsuhisa
等离子体医学是一个很有吸引力的新的研究领域，但等离子体修饰水溶液中的生物分子的原理仍然是难以捉摸的。在这项研究中，我们研究了大气压冷等离子体对水溶液中20种天然氨基酸的化学效应。高分辨率质谱分析显示，等离子体处理后观察到14种氨基酸的化学修饰：（i）酪氨酸、苯丙氨酸和色氨酸中芳香环的羟基化和硝化;（ii）半胱氨酸中巯基的磺化和二硫键形成;（iii）蛋氨酸的磺基氧化和（iv）组氨酸和脯氨酸中五元环的酰胺化和开环。使用20种氨基酸的竞争性反应实验表明，含硫和芳香族氨基酸优先减少等离子体处理。这些数据为阐明生物医学等离子体应用中蛋白质失活的机制提供了基础信息。
Plasma medicine is an attractive new research area, but the principles of plasma modification of biomolecules in aqueous solution remain elusive. In this study, we investigated the chemical effects of atmospheric-pressure cold plasma on 20 naturally occurring amino acids in aqueous solution. High-resolution mass spectrometry revealed that chemical modifications of 14 amino acids were observed after plasma treatment: (i) hydroxylation and nitration of aromatic rings in tyrosine, phenylalanine and tryptophan; (ii) sulfonation and disulfide linkage formation of thiol groups in cysteine; (iii) sulfoxidation of methionine and (iv) amidation and ring-opening of five-membered rings in histidine and proline. A competitive reaction experiment using 20 amino acids demonstrated that sulfur-containing and aromatic amino acids were preferentially decreased by the plasma treatment. These data provide fundamental information for elucidating the mechanism of protein inactivation for biomedical plasma applications.