Wafer-scale mitochondrial membrane potential assays.
Wafer-scale mitochondrial membrane potential assays.
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DOI：
10.1039/c2lc40086c
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发表时间：
2012-08-07
期刊：
影响因子：
6.1
通讯作者：
Burke PJ
中科院分区：
文献类型：
--
作者：
Lim TS;Davila A Jr;Zand K;Wallace DC;Burke PJ
据报道，线粒体代谢和生物物理参数与退行性疾病和衰老过程相关。为了评估这些生化参数，目前的技术需要几百毫克的分离的线粒体用于功能测定。在这里，我们展示了可制造的晶圆级线粒体功能测定芯片实验室设备，其需要的线粒体蛋白质量比当前的测定少三个数量级，并使用新的制造工艺和材料集成到4”标准硅晶圆上。本文研究并比较了人HeLa细胞系（Heb7A）、人骨肉瘤细胞系（143b）和小鼠骨骼肌组织中分离的线粒体膜电位的变化。与第一代技术相比，在此开发的第二代集成实验室芯片系统显示出增强的结构耐久性和再现性，同时增加了对线粒体膜电位变化的敏感性。我们设想这个系统是一个伟大的候选人，以取代目前的线粒体检测系统。
It has been reported that mitochondrial metabolic and biophysical parameters are associated with degenerative diseases and the aging process. To evaluate these biochemical parameters, current technology requires several hundred milligrams of isolated mitochondria for functional assays. Here, we demonstrate manufacturable wafer-scale mitochondrial functional assay lab-on-a-chip devices, which require mitochondrial protein quantities three orders of magnitude less than current assays, integrated onto 4” standard silicon wafer with new fabrication processes and materials. Membrane potential changes of isolated mitochondria from various well-established cell lines such as human HeLa cell line (Heb7A), human osteosarcoma cell line (143b) and mouse skeletal muscle tissue were investigated and compared. This second generation integrated lab-on-a-chip system developed here shows enhanced structural durability and reproducibility while increasing the sensitivity to changes in mitochondrial membrane potential by an order of magnitude as compared to first generation technologies. We envision this system to be a great candidate to substitute current mitochondrial assay systems.
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影响因子：
2.9
作者：
Labajova, A;Vojtiskova, A;Houstek, J
通讯作者：
Houstek, J
影响因子：
6.1
作者：
Lim, Tae-Sun;Davila, Antonio;Burke, Peter
通讯作者：
Burke, Peter
影响因子：
7.2
作者：
Galluzzi, Lorenzo;Zamzami, Naoufal;Kroemer, Guido
通讯作者：
Kroemer, Guido
影响因子：
4
作者：
Solaini, Giancarlo;Sgarbi, Gianluca;Baracca, Alessandra
通讯作者：
Baracca, Alessandra
影响因子：
2.4
作者：
KAMO, N;MURATSUGU, M;KOBATAKE, Y
通讯作者：
KOBATAKE, Y