A Stable-Isotope Dilution GC-MS Approach for the Analysis of DFRC (Derivatization Followed by Reductive Cleavage) Monomers from Low-Lignin Plant Materials
A Stable-Isotope Dilution GC-MS Approach for the Analysis of DFRC (Derivatization Followed by Reductive Cleavage) Monomers from Low-Lignin Plant Materials
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DOI：
10.1021/jf506221p
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发表时间：
2015-03-18
影响因子：
6.1
通讯作者：
Bunzel, Mirko
中科院分区：
文献类型：
--
作者：
Schaefer, Judith;Urbat, Felix;Bunzel, Mirko
衍生化后还原裂解（DFRC）方法是一种成熟的表征植物材料木质素组成的工具。然而，原方法的应用，特别是DFRC单体的色谱测定在低木质素食品中存在问题。为了克服这些问题，开发并验证了改进的样品清理和稳定同位素稀释方法。为了定量二乙酰化DFRC单体，合成了相应的六氘化类似物并作为内标。通过使用选择离子监测模式，矩阵相关的干扰可以最小化，从而获得更高的选择性和灵敏度。将改进后的方法应用于四种低木质素样品。胡萝卜纤维中的木质素被归类为富含愈创木酰，而萝卜、梨和芦笋纤维中的木质素被归类为平衡木质素（愈创木酰/丁香基比=1-2）。
The derivatization followed by reductive cleavage (DFRC) method is a well-established tool to characterize the lignin composition of plant materials. However, the application of the original procedure, especially the chromatographic determination of the DFRC monomers, is problematic for low-lignin foods. To overcome these problems a modified sample cleanup and a stable-isotope dilution approach were developed and validated. To quantitate the diacetylated DFRC monomers, their corresponding hexadeuterated analogs were synthesized and used as internal standards. By using the selected-ion monitoring mode, matrix-associated interferences can be minimized resulting in higher selectivity and sensitivity. The modified method was applied to four low-lignin samples. Lignin from carrot fibers was classified as guaiacyl-rich whereas the lignins from radish, pear, and asparagus fibers where classified as balanced lignins (guaiacyl/syringyl ratio=1-2).