Molecular cloning and functional expression of a serotonin receptor from Caenorhabditis elegans
Molecular cloning and functional expression of a serotonin receptor from Caenorhabditis elegans
复制标题
DOI：
10.1007/bf02736863
复制
发表时间：
1997-02-01
影响因子：
3.1
通讯作者：
McCombie, WR
中科院分区：
文献类型：
--
作者：
Olde, B;McCombie, WR
编码5-羟色胺受体的cDNA已从秀丽隐杆线虫混合阶段cDNA文库中分离。命名为5 HT-Ce的线虫5-羟色胺受体在鼠Ltk-细胞中永久表达，在那里它介导腺苷酸环化酶衰减。序列分析和药理学特征表明它不仅与果蝇和果蝇的5 HT受体有关，而且与哺乳动物的5 HT 1a受体有关。5 HT-Ce基因不映射接近任何已知的多巴胺能突变的位置。
A cDNA encoding a serotonin receptor has been isolated from a Caenorhabditis elegans mixed stage cDNA library. The nematode serotonin receptor, designated 5HT-Ce, was permanently expressed in murine Ltk- cells, where it mediates adenylate cyclase attenuation. Sequence analysis and the pharmacological profiles demonstrate its relatedness not only to Drosophila and Lymnae 5HT receptors but also to mammalian 5HT1a receptors. The 5HT-Ce gene does not map close to the position of any known serotonergic mutations.