Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese
Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese
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从西藏牦牛 (Bos grunniens) 中分离出的粪肠球菌 JF85 和粪肠球菌 Y83 用于青贮狼尾草的特性
DOI：
10.1016/j.biortech.2018.02.070
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发表时间：
2018-06-01
影响因子：
11.4
通讯作者：
Shao, Tao
中科院分区：
文献类型：
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作者：
Li, Junfeng;Yuan, Xianjun;Shao, Tao
从藏牦牛瘤胃中分离到2株具有纤维素降解能力的细菌，经鉴定为粪肠球菌（Enterococcus faecalis，JF 85）和屎肠球菌（Enterococcus faecium，Y 83）。分离物分别在15至55 ℃的温度和pH 3.0-7.0的范围内生长良好。将两株菌分别与植物乳杆菌（Lactobacillusplantarum，Lp）和对照接种于中华狼尾草青贮饲料中，培养90 d。与不添加添加剂的青贮饲料（对照）相比，所有接种剂均增加乳酸含量，降低pH和木质纤维素含量。JF 85 +Lp和Y83+Lp青贮料的pH值最低，乳酸含量最高，木质纤维素含量下降最大。单独或与Lp组合的分离物与对照相比显著增加WSC、单糖和二糖含量。复合添加有效地提高了狼尾草青贮饲料的酶解，表现为较高的葡萄糖产量和纤维素转化率。复合添加剂青贮狼尾草是一种适宜的能源作物制糖前的贮藏和预处理方法。
Two bacteria strains with cellulolytic potential isolated from Tibetan yak (Bos grunniens) rumen were identified as Enterococcus faecalis (JF85) and Enterococcus faecium (Y83). Isolates grow well within a range of temperature 15 to 55 degrees C and pH 3.0-7.0, respectively. Two strains were inoculated with or without Lactobacillus plantarum (Lp) to Pennisetum sinese silage for 90 days. All inoculants increased lactic acid content, decreased pH and lignocellulose contents compared with silage without additives (control). The lowest pH, highest lactic acid and largest reduction in lignocellulose contents were observed in JF85+Lp and Y83+Lp silages. Isolates alone or in combination with Lp significantly increased WSC, mono-and disaccharides contents as compared to the control. Combined addition efficiently improved enzymatic hydrolysis of Pennisetum sinese silage, indicated by higher glucose yield and cellulose convertibility. Pennisetum sinese ensiled with combined additives is a suitable storage and pretreatment method prior to sugars production from energy crop.