Extracellular matrix proteins and myofibroblasts in granulomas of sarcoidosis, atypical mycobacteriosis, and tuberculosis of the lung
Extracellular matrix proteins and myofibroblasts in granulomas of sarcoidosis, atypical mycobacteriosis, and tuberculosis of the lung
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DOI：
10.1016/j.humpath.2006.07.001
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发表时间：
2007-01-01
期刊：
影响因子：
3.3
通讯作者：
Somi, Ylermi
中科院分区：
文献类型：
--
作者：
Kaarteenaho-Wiik, Riitta;Sademies, Outi;Somi, Ylermi
结节病、非典型分枝杆菌病和结核病是人类肺部的常见疾病，其典型特征是形成肉芽肿。肉芽肿的结构尚未完全阐明。我们研究了结节病、非典型分枝杆菌病和肺结核肉芽肿中生腱蛋白-C、胶原蛋白 I 和 III 的前体蛋白的表达以及肌成纤维细胞的存在。使用肌腱蛋白-C 抗体以及胶原蛋白 I 和 III 的氨基末端前肽，通过免疫组织化学分析了 25 个肺组织学样本。为了识别肉芽肿中的肌成纤维细胞型细胞，还用抗 α-平滑肌肌动蛋白、波形蛋白和结蛋白的抗体对切片进行染色。在每种情况下，生腱蛋白-C 以及 I 型和 III 型胶原蛋白的前体蛋白均在肉芽肿周围表达。 I 型胶原蛋白前体蛋白也在其中表达。在结核病和非典型分枝杆菌病中，生腱蛋白-C 和 I 型胶原前体蛋白的表达强于结节病。划分肉芽肿的细胞，因此与生腱蛋白-C 和两种胶原前体共定位的细胞，α-平滑肌肌动蛋白和波形蛋白呈阳性，这表明这些细胞是肌成纤维细胞。正如α-平滑肌肌动蛋白染色所表明的，它们在结核病和非典型分枝杆菌病中也更丰富地存在。我们得出的结论是，生腱蛋白-C 以及 I 型和 III 型胶原蛋白的前体蛋白在结节病、非典型分枝杆菌病和肺结核的肉芽肿周围表达；此外，它们的表达与肌成纤维细胞的表达共定位。我们的结果进一步表明，结核病和非典型分枝杆菌病的纤维形成和基质更新比结节病更强。 (c) 2007 Elsevier Inc. 保留所有权利。
Sarcoidosis, atypical mycobacteriosis, and tuberculosis are common diseases of human lung with a typical feature of formation of granulomas. The structure of granulomas has not been elucidated completely. We studied the expression of tenascin-C, precursor proteins of collagens I and III, and the presence of myofibroblasts in granulomas of sarcoidosis, atypical mycobacteriosis, and tuberculosis of human lung. Twenty-five histologic samples of lung were analyzed by immunohistochemistry using antibodies to tenascin-C and aminoterminal propeptides of collagens I and Ill. To identify the myofibroblast-type cells in granulomas, the sections were also stained with antibodies against alpha-smooth muscle actin, vimentin, and desmin. In every case, tenascin-C and precursor proteins of collagens I and Ill were expressed around granulomas. Precursor protein of collagen I was expressed also within them. In tuberculosis and atypical mycobacteriosis, expression of tenascin-C and precursor protein of collagen I was stronger than in sarcoidosis. The cells demarcating granulomas and, thus, colocalizing with tenascin-C and both collagen precursors were positive for alpha-smooth muscle actin and vimentin, which suggests that these cells are myofibroblasts. They were also more abundantly present in tuberculosis and atypical mycobacteriosis, as suggested by a-smooth muscle actin staining. We concluded that tenascin-C and precursor proteins of collagens I and III are expressed around granulomas in sarcoidosis, atypical mycobacteriosis, and tuberculosis of the lung; and furthermore, their expression colocalize with the expression of myofibroblasts. Our results further point to the fact that fibrogenesis and matrix turnover is stronger in tuberculosis and atypical mycobacteriosis than in sarcoidosis. (c) 2007 Elsevier Inc. All rights reserved.