microRNA-195 promotes apoptosis and suppresses tumorigenicity of human colorectal cancer cells
microRNA-195 promotes apoptosis and suppresses tumorigenicity of human colorectal cancer cells
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DOI：
10.1016/j.bbrc.2010.08.046
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发表时间：
2010-09-17
影响因子：
3.1
通讯作者：
Du, Xiaohui
中科院分区：
文献类型：
--
作者：
Liu, Lin;Chen, Lin;Du, Xiaohui
去调控的microRNAs及其在癌症发展中的作用引起了人们的极大关注。在这项研究中，我们分析了miR-195在结直肠癌发病机制中的作用，因为以前的报道已经表明它参与了一些其他类型的癌症。通过比较81例结直肠癌组织和配对的非肿瘤黏膜组织中miR-195的表达，我们发现miR-195在癌组织中表达下调。在结直肠癌细胞株HT29和LoVo中修复miR-195可以降低细胞存活率，促进细胞凋亡，抑制致瘤性。此外，miR-195还直接靶向了重要的抗凋亡基因Bcl2，并进一步提示miR-195主要通过靶向Bcl2的表达来发挥其促凋亡作用。综上所述，我们的研究提供了miR-195在结直肠癌发病机制中的重要作用，并暗示了其在肿瘤治疗中的潜在应用。(C)2010 Elsevier Inc.保留所有权利。
Deregulated microRNAs and their roles in cancer development have attracted much attention. In the present study, we analyzed the roles of miR-195 in colorectal cancer pathogenesis, as its participation in some other types of cancer has been suggested by previous reports. By comparing miR-195 expression in 81 human colorectal cancer tissues and matched non-neoplastic mucosa tissues, we found that miR-195 was downregulated in cancer tissues. And restoration of miR-195 in colorectal cancer cell lines HT29 and LoVo could reduce cell viability, promote cell apoptosis and suppress tumorigenicity. Moreover, important antiapoptotic Bcl-2 was identified to be directly targeted by miR-195, and miR-195 was further suggested to exert its proapoptotic function mainly through targeting Bcl-2 expression. Taken together, our study provides important roles of miR-195 in colorectal cancer pathogenesis and implicates its potential application in cancer therapy. (C) 2010 Elsevier Inc. All rights reserved.