In situ distribution in different cell types of nuclear glycoconjugates detected by two lectins.
In situ distribution in different cell types of nuclear glycoconjugates detected by two lectins.
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两种凝集素检测到的核糖缀合物在不同细胞类型中的原位分布。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
M. Bouteille
中科院分区：
文献类型：
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作者：
A. Seve;J. Hubert;D. Bouvier;C. Masson;G. Géraud;M. Bouteille
核糖缀合物原位检测两个凝集素-伴刀豆球蛋白A和小麦胚芽凝集素-组织切片包埋在亲水性树脂乙二醇甲基丙烯酸酯。将这些凝集素与异硫氰酸荧光素缀合用于荧光显微镜，并用铁蛋白标记用于电子显微镜。本研究选择胎生蜥蜴的卵巢滤泡，因为它可以在同一切片上观察到四种类型的细胞核，具有不同的超微结构和生理功能。在这种材料中，发现所有的核仁都含有高浓度的两种凝集素受体。位于染色质和核仁中的受体的分布被观察到在相同类型的细胞核内变化，这取决于所使用的凝集素。我们的一些研究结果表明，小麦胚芽凝集素标记的增加可能取决于核糖体前体的生产增加。
Nuclear glycoconjugates were detected in situ by two lectins--Concanavalin A and Wheat germ agglutinin--on tissue sections embedded in the hydrophilic resin glycol methacrylate. These lectins were conjugated with fluorescein isothiocyanate for fluorescence microscopy, and labelled with ferritin for electron microscopy. The ovarian follicle of the lizard Lacerta vivipara was chosen for this study, because it enables four types of nuclei, with different ultrastructures and physiology to be observed on the same section. In this material, all the nucleoli were found to contain a high concentration of receptors for both lectins. The distribution of the receptors located in the chromatin and nucleoli was observed to vary within the same type of nucleus, depending on the lectin used. Some of our results suggest that increased labelling by Wheat germ agglutinin might depend on increased production of ribosomal precursors.