Method for evaluating mutagenicity of water. I. A new method of preparing samples for mutagenicity test.
Method for evaluating mutagenicity of water. I. A new method of preparing samples for mutagenicity test.
复制标题
评价水致突变性的方法。
DOI：
10.1016/0048-9697(88)90136-2
复制
发表时间：
1988
影响因子：
9.8
通讯作者：
T. Shinome
中科院分区：
文献类型：
--
作者：
K. Urano;N. Haga;F. Emoto;T. Shinome
采用两种新型优质树脂吸附剂，建立了一种简便、定量的水致突变性试验样品制备方法。CSP800是一种小粒径、高孔隙度的聚苯乙烯树脂，CHPA25是一种小粒径、高孔隙度的阴离子交换树脂。CSP800对水中非离子型化合物的吸附效果优于常规树脂，而CHPA25对阴离子型化合物的吸附效果优于常规树脂。在550 ml h - 1条件下，将pH为5的样品水（101）串联进两根柱，一根柱含有5ml CSP800，另一根柱含有2ml CHPA25。吸附物质随后用CSP800中的DMSO和CHPA25中的4nnano3在10 ml h−1下解吸。用总三卤甲烷（THM）、总有机卤素（TOX）和260 nm吸光度等不同指标测定的各种有机物质，与常规方法相比，该方法可以更有效、更方便地回收。
A sample preparation method for a convenient and quantitative mutagenicity test of water was developed using two new superior resinous adsorbents. One, CSP800, is a small particle size and highly porous polystyrene resin, and the other, CHPA25, is a small particle size and highly porous anion-exchange resin. CSP800 adsorbed more nonionic compounds from water than conventional resins, and CHPA25 adsorbed anionic compounds not adsorbed by conventional resins. Sample water (101) at pH 5 was fed at 550 ml h−1into two columns in series, one containing 5 ml of CSP800 and the other 2 ml of CHPA25. The adsorbed substances were subsequently desorbed by DMSO from CSP800 and by 4NNaNO3from CHPA25, at 10 ml h−1. Various organic substances, which were measured by different indices such as total trihalomethanes (THM), total organic halogen (TOX) and the absorbence at 260 nm, could be recovered by this method much more efficiently and conveniently than by conventional methods.