Blood-brain barrier permeability of Gualou Guizhi granules and neuroprotective effects in ischemia/reperfusion injury
Blood-brain barrier permeability of Gualou Guizhi granules and neuroprotective effects in ischemia/reperfusion injury
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DOI：
10.3892/mmr.2015.3520
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发表时间：
2015-07-01
影响因子：
3.4
通讯作者：
Que, Jinhua
中科院分区：
文献类型：
--
作者：
Li, Huang;Ye, Miao;Que, Jinhua
本研究旨在观察瓜络桂枝颗粒对正常大鼠和大鼠脑缺血再灌注损伤模型血脑屏障通透性的影响，并观察其神经保护作用。建立了灵敏的高效液相色谱-四极杆飞行时间-质谱法测定血浆和脑组织中GLGZG组分的方法。以大鼠大脑中动脉闭塞(MCAO)为模型，瓜氨酸、没食子酸、没食子酸、芍药苷、甘草苷、甘草苷、异甘草素、甘草酸迅速进入血流。瓜氨酸、白花苷、芍药苷、甘草素、异甘草素和甘草酸也通过血脑屏障进入MCAO大鼠脑组织，而正常大鼠脑组织中未检测到异甘草素和甘草酸。观察GLGZG对大脑中动脉阻塞大鼠的神经保护作用。大鼠局灶性脑缺血再灌注2 h后灌胃给予GLGZG，可减少神经功能缺失和脑梗塞体积，减轻脑组织病理改变，对脑缺血损伤有明显的保护作用。综上所述，GLGZG的某些成分通过血脑屏障，尤其是在脑缺血损伤后，这可能是治疗脑缺血损伤的有效方法。
The present study aimed to estimate the blood-brain barrier (BBB) permeability of Gualou Guizhi granules (GLGZG) in normal rats and in rat models of ischemia/reperfusion (I/R) injury, and to examine the neuroprotective effects of GLGZG. A sensitive high-performance liquid chromatography-quadrupole-time of flight-mass spectrometry analytical method was developed to determinate the components of GLGZG in the plasma and brain tissue. Middle cerebral artery occlusion (MCAO) in rats served as a model of in vivo I/R. Citrulline, gallic acid, albiflorin, peoniflorin, liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, isoliquiritigenin and glycyrrhizinic acid rapidly passed into the bloodstream. Citrulline, albiflorin, peoniflorin, liquiritin apioside, liquiritin, liquiritigenin, isoliquiritigenin and glycyrrhizinic acid also passed the BBB and reached the brain tissue of MCAO rats, while isoliquiritigenin and glycyrrhizinic acid were not detected in the brain tissue of the normal rats. The potential neuroprotective effect of GLGZG was determined in MCAO rats. The intragastric administration of GLGZG following reperfusion of rats for 2 h decreased the neurological defects and infarction volume, attenuated pathological changes of brain tissue and exerted a significant protective effect in cerebral ischemia injury. In conclusion, certain components of GLGZG passed through the BBB, particularly following cerebral ischemia injury, and this may be therapeutically effective for the treatment of cerebral ischemia injury in the human brain.