Removal of Listeria monocytogenes Biofilms from Stainless Steel by Use of Ultrasound and Ozone
Removal of Listeria monocytogenes Biofilms from Stainless Steel by Use of Ultrasound and Ozone
复制标题
DOI：
10.4315/0362-028x-72.6.1306
复制
发表时间：
2009-06-01
影响因子：
2
通讯作者：
Hao Feng
中科院分区：
文献类型：
--
作者：
Baumann, Adam R.;Martin, Scott E.;Hao Feng
本研究的目的是确定功率超声和臭氧单独使用，并在串联，从不锈钢芯片的单核细胞增生李斯特菌生物膜的去除效果。不锈钢片接种L.单核细胞增多症在距离生物膜芯片2.54 cm处施加功率超声（20 kHz，100%振幅，120 W）30或60 s，同时将其浸没在250 ml无菌磷酸钾缓冲液（pH 7.0）中。臭氧也以0.25、0.5或1.0 ppm的浓度循环通过含有生物膜芯片的250 ml磷酸钾缓冲液30或60 s。功率超声和臭氧氧化也被用于串联测试它们的组合效果。单独的每种处理都导致可恢复细胞的显著减少，其中功率超声是最有效的（60秒后减少3.8-log CFU/ml）。对于臭氧与功率超声治疗的组合，减少显著（P < 0.05）高于单独治疗。当使用0.5ppm的臭氧浓度时，在该组合处理60秒后没有可恢复的细胞（7.31-log CFU/ml减少）。这些结果表明，功率超声和臭氧氧化的组合可能是一种有效的处理从不锈钢食品接触表面的生物膜去除。
The objective of this study was to determine the efficacy of power ultrasound and ozonation used individually, and in tandem, for the removal of Listeria monocytogenes biofilms from stainless steel chips. Stainless steel chips were inoculated with L. monocytogenes. Power ultrasound (20 kHz, 100% amplitude, 120 W) was applied for 30 or 60 s at a distance of 2.54 cm from a biofilm chip while it was submerged in 250 ml of sterile potassium phosphate buffer (pH 7.0). Ozone was cycled through the 250 ml of potassium phosphate buffer containing the biofilm chip also for 30 or 60 s at concentrations of 0.25, 0.5, or 1.0 ppm. Power ultrasound and ozonation were also used in tandem for testing of their combined effect. Each of the treatments alone resulted in a significant reduction in recoverable cells, with power ultrasound being the most effective (3.8-log CFU/ml reduction after 60 s). For the ozone in combination with power ultrasound treatment, reductions were significantly (P < 0.05) higher than by either treatment alone. There were no recoverable cells after 60 s of this combined treatment when an ozone concentration of 0.5 ppm was used (7.31-log CFU/ml reduction). These results indicated that the combination of power ultrasound and ozonation may be an effective treatment for biofilm removal from stainless steel food contact surfaces.