Enhanced tolerance of the transgenic potato plants overexpressing Cu/Zn superoxide dismutase to low temperature
Enhanced tolerance of the transgenic potato plants overexpressing Cu/Zn superoxide dismutase to low temperature
复制标题
过表达铜/锌超氧化物歧化酶的转基因马铃薯植株的低温耐受性增强
DOI：
10.1016/j.scienta.2019.108949
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发表时间：
2020-02-05
影响因子：
4.3
通讯作者：
Si, Huaijun
中科院分区：
文献类型：
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作者：
Che, Yuzhang;Zhang, Ning;Si, Huaijun
低温胁迫是造成植物特别是粮食作物危害的主要因素之一。Cu/Zn超氧化物歧化酶在清除环境胁迫引起的活性氧（ROS）过程中起重要作用。为了研究转基因马铃薯植株是否提高了对低温的耐受性，本研究通过根癌农杆菌介导法，在CaMV 35 S启动子控制下，将StSOD 1基因在马铃薯中过量表达。结果表明，在4 ℃处理48 h后，过表达StSOD 1的转基因马铃薯品系（OE品系）的超氧化物歧化酶（SOD）活性比非转基因植株（NT品系）提高了1.38倍。另一方面，抑制StSOD 1表达的转基因马铃薯品系（RNAi品系）的活性与NT品系相比降低。此外，通过测定低温处理过程中丙二醛（MDA）含量，研究了SOD活性增加对膜脂过氧化的影响。结果表明，4 ℃处理48 h后，NT和RNAi株系的MDA含量分别增加了2.02倍和1.78倍。相反，OE系仅显示出小的变化并且低于NT系。同时，低温处理后OE系的过氧化物酶（POD）和过氧化氢酶（CAT）活性也比NT系有所提高。通过对表型的观察，本研究发现NT系和RNAi系叶片萎蔫严重，而OE系叶片萎蔫程度较轻。这些结果表明，可以利用基因工程技术调控植物体内抗氧化酶的活性，提高植物对低温胁迫的耐受性。
Low temperature stress is one of the major factors for damage in plants, especially in food crops. Cu/Zn superoxide dismutase plays an important role in the processes of scavenging reactive oxygen species (ROS) caused by environmental stresses. In order to study transgenic potato plants whether improved tolerance to low temperature, in this research, StSOD1 gene was overexpressed under the control of CaMV 35S promoter through Agrobacterium tumefaciens-mediated transformation in potato. The results showed that after 4 degrees C treatment for 48 h, superoxide dismutase (SOD) activity of the transgenic potato lines (OE lines) overexpressing StSOD1 was 1.38-fold enhanced compared to the non-transgenic plants (NT lines). On the other hand, the activity of the transgenic potato lines (RNAi lines) that inhibiting expression of StSOD1 decreased compared with NT lines. In addition, the effect of increased SOD activity on lipid peroxidation was determined by measuring malondialdehyde (MDA) contents in plants during cold treatment. The result showed that MDA content increased by 2.02-fold and 1.78-fold in NT lines and RNAi lines after 4 degrees C treatment for 48 h, respectively. In contrast, the OE lines showed only small changes and were lower than the NT lines. Meanwhile, the activity of peroxidase (POD) and catalase (CAT) were also enhanced in OE lines compared with NT lines after cold treatment. Through observing the phenotype, importantly, this research found that the leaves of NT lines and RNAi lines have wilted severely while the OE lines were slightly affected. In all, these results indicated that genetic engineering technology could be used to regulate the antioxidant enzymes activity in plants to improve tolerance to cold stress.