IMMUNOLOCALIZATION OF NA+, K+-ATPASE, CA++-ATPASE, CALCIUM-BINDING PROTEINS, AND CARBONIC-ANHYDRASE IN THE GUINEA-PIG INNER-EAR
IMMUNOLOCALIZATION OF NA+, K+-ATPASE, CA++-ATPASE, CALCIUM-BINDING PROTEINS, AND CARBONIC-ANHYDRASE IN THE GUINEA-PIG INNER-EAR
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DOI：
10.3109/00016489409126037
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发表时间：
1994-03-01
影响因子：
1.4
通讯作者：
KIMURA, RS
中科院分区：
文献类型：
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作者：
ICHIMIYA, I;ADAMS, JC;KIMURA, RS
用免疫组织化学方法研究了豚鼠内耳石蜡切片中Na ~+，K ~+-ATP酶、Ca ~（++）-ATP酶、碳酸酐酶和钙结合蛋白的分布。血管纹的边缘细胞、螺旋韧带的II型纤维细胞以及缘上和缘上区域的细胞呈Na+，K+-ATP酶阳性。螺旋韧带I型纤维细胞Ca++-ATP酶、碳酸酐酶、钙调素和骨桥蛋白阳性。前庭系统暗细胞Na+，K+-ATP酶阳性。然而，这些细胞和上皮下纤维细胞的Ca+-ATP酶，碳酸酐酶，和钙结合蛋白呈阴性。内淋巴囊中部和远端上皮细胞Na+，K+-ATP酶呈阳性反应，但其阳性反应较纹状上皮细胞弱。Na+，K+-ATP酶阳性的内淋巴囊细胞，其Ca++-ATP酶和钙结合蛋白也呈阳性，而碳酸酐酶呈阴性。螺旋韧带的I型纤维细胞中存在Ca++-ATP酶和钙结合蛋白，表明这些细胞参与介导Ca++调节。内淋巴囊上皮细胞Na ~+，K ~+-ATP酶水平较低，Ca ~（++）-ATP酶与钙结合蛋白共存，表明内淋巴囊上皮细胞在离子转运中具有不同于血管纹和前庭暗细胞的独特作用。
The distribution of Na+, K+-ATPase, Ca++-ATPase, carbonic anhydrase, and calcium-binding proteins were investigated immunohistochemically in paraffin sections of guinea pig inner ears. Marginal cells of the stria vascularis, type II fibrocytes of the spiral ligament, and cells in supralimbal and suprastrial regions, were positive for Na+, K+-ATPase. Type I fibrocytes of the spiral ligament were positive for Ca++-ATPase, carbonic anhydrase, calmodulin and osteopontin. In the vestibular system, dark cells were positive for Na+, K+-ATPase. However, these cells and subepithelial fibrocytes were negative for Ca+-ATPase, carbonic anhydrase, and the calcium-binding proteins. In the endolymphatic sac, epithelial cells in intermediate and distal portions were positive for Na+, K+-ATPase, but the reaction was less than that in the stria. The same endolymphatic sac cells that were positive for Na+, K+-ATPase were also positive for Ca++-ATPase and calcium-binding proteins, but negative for carbonic anhydrase. The presence of Ca++-ATPase and calcium-binding proteins in the type I fibrocytes of the spiral ligament suggests that these cells are involved in mediating Ca++ regulation. Lower levels of Na+, K+-ATPase and the co-existence of Ca++-ATPase and calcium-binding proteins in the epithelial cells of the endolymphatic sac indicate that these cells have a distinctive role in ion transport that is different from that of the cells of the stria vascularis and vestibular dark cells.