Length measurement: new developments in neurostereology and 3D imagery
Length measurement: new developments in neurostereology and 3D imagery
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DOI：
10.1016/s0891-0618(00)00074-0
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发表时间：
2000-10-01
影响因子：
2.8
通讯作者：
Mouton, PR
中科院分区：
文献类型：
--
作者：
Calhoun, ME;Mouton, PR
线性生物结构的量化在神经科学中有重要的应用；例如，特定脑结构内神经递质特异性轴突的长度或树突的长度。然而，直到最近，在应用体视学工具对组织切片上的对象长度进行无偏估计方面还存在实际限制。最近有效的新方法的发展使理论上无偏见的抽样和估计技术得以更广泛地应用，这些技术不需要以前方法的假设和模型。在本文中，我们概述了组织切片上生物物体全长估计的历史背景和最新进展，包括一种利用新开发的方法估计胆碱能纤维长度的实用方法。这些较新的方法还利用了三维图像数据集和虚拟探针，这些技术可能在定量形态测量中有更广泛的应用。(C)2000 Elsevier Science B.V.保留所有权利。
Quantification of linear biological structures has important applications in neuroscience; for example, the length of neurotransmitter-specific axonal innervation or length of dendritic processes within particular brain structures. Until recently, however, there have been practical limitations in the application of stereological tools for the unbiased estimation of object length on tissue sections. The recent development of efficient new approaches allows for the wider application of theoretically unbiased sampling and estimation techniques that are devoid of the assumptions and models of earlier methods. In this review, we outline the historical background and recent advances in the estimation of total length for biological objects on tissue sections, including a practical method to estimate the length of cholinergic fibers using newly developed methods. These newer methods also take advantage of three-dimensional image datasets and virtual probes, techniques that may have wider application in quantitative morphometry. (C) 2000 Elsevier Science B.V. All rights reserved.