Exposure to phages has little impact on the evolution of bacterial antibiotic resistance on drug concentration gradients.
Exposure to phages has little impact on the evolution of bacterial antibiotic resistance on drug concentration gradients.
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暴露于噬菌体对药物浓度梯度上细菌抗生素耐药性的演变影响不大
DOI：
10.1111/eva.12136
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发表时间：
2014-03
影响因子：
4.1
通讯作者：
Zhang QG
中科院分区：
文献类型：
--
作者：
Zhang QG
最近提倡使用抗生素治疗细菌性病原体，作为抗生素治疗的替代方案。在这里，我们测试了一个假设，即用抗生素处理的细菌可能显示出更有限的抗生素耐药性进化，因为对抗生素耐药性的适应性成本可能会增加抗生素耐药性的适应性成本，进一步降低抗生素耐药性细菌的生长性能。我们通过研究暴露于噬菌体和无噬菌体的荧光假单胞菌培养物在单一药物（包括头孢噻肟、氯霉素和卡那霉素）浓度梯度上的演变来做到这一点。在药物治疗期间，细菌抗生素抗性水平随着时间的推移而增加，并且不受噬菌体治疗的影响。暴露于氯吡格雷不会导致抗生素耐药细菌的生长减慢，尽管它会导致抗生素敏感细菌的生长减慢。我们观察到药物使用终止后抗生素耐药性的显著逆转，并且逆转率不受噬菌体处理的影响。结果表明，噬菌体耐药性引起的适应性成本不太可能成为异质药物环境中细菌抗生素耐药性演变的重要限制因素。需要进一步研究抗生素耐药性的适应性成本与其他因素的相互作用。
The use of phages for treating bacterial pathogens has recently been advocated as an alternative to antibiotic therapy. Here, we test a hypothesis that bacteria treated with phages may show more limited evolution of antibiotic resistance as the fitness costs of resistance to phages may add to those of antibiotic resistance, further reducing the growth performance of antibiotic‐resistant bacteria. We did this by studying the evolution of phage‐exposed and phage‐free Pseudomonas fluorescens cultures on concentration gradients of single drugs, including cefotaxime, chloramphenicol, and kanamycin. During drug treatment, the level of bacterial antibiotic resistance increased through time and was not affected by the phage treatment. Exposure to phages did not cause slower growth in antibiotic‐resistant bacteria, although it did so in antibiotic‐susceptible bacteria. We observed significant reversion of antibiotic resistance after drug use being terminated, and the rate of reversion was not affected by the phage treatment. The results suggest that the fitness costs caused by resistance to phages are unlikely to be an important constraint on the evolution of bacterial antibiotic resistance in heterogeneous drug environments. Further studies are needed for the interaction of fitness costs of antibiotic resistance with other factors.
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影响因子：
5.2
作者：
Enne, VI;Delsol, AA;Bennett, PM
通讯作者：
Bennett, PM
影响因子：
3.4
作者：
Chanishvili, N;Chanishvili, T;Barrow, PA
通讯作者：
Barrow, PA
影响因子：
8.6
作者：
Greulich, Philip;Waclaw, Bartlomiej;Allen, Rosalind J.
通讯作者：
Allen, Rosalind J.
DOI：
10.1073/pnas.1117716109
发表时间：
2012-07-03
影响因子：
11.1
作者：
Hermsen, Rutger;Deris, J. Barrett;Hwa, Terence
通讯作者：
Hwa, Terence
影响因子：
4.9
作者：
Bailey, MJ;Lilley, AK;Ellis, RJ
通讯作者：
Ellis, RJ