Platelet adhesion in the sinusoid caused hepatic injury by neutrophils after hepatic ischemia reperfusion
Platelet adhesion in the sinusoid caused hepatic injury by neutrophils after hepatic ischemia reperfusion
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DOI：
10.3109/09537101003637265
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发表时间：
2010-01
期刊：
影响因子：
3.3
通讯作者：
Sugiru Pak;T. Kondo;Y. Nakano;S. Murata;K. Fukunaga;T. Oda;R. Sasaki;N. Ohkohchi
中科院分区：
文献类型：
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作者：
Sugiru Pak;T. Kondo;Y. Nakano;S. Murata;K. Fukunaga;T. Oda;R. Sasaki;N. Ohkohchi
肝脏缺血再灌注损伤是肝脏外科最严重的并发症之一。在I/R中，活化的枯否细胞引起血小板粘附于窦内皮以及中性粒细胞，并引起肝功能障碍。本研究的目的是通过活体显微镜（IVM）评价I/R后肝脏微循环中血小板动力学，并阐明血小板粘附与中性粒细胞活化之间的关系。将雄性Sprague-Dawley（SD）大鼠分为两组：对照组（给予生理盐水）和西维来司他组，其中在I/R之前通过西维来司他抑制中性粒细胞活化。IVM观察血窦内粘附血小板数，直至I/R后120分钟。取肝组织和血液样本进行组织学检查、肝酶和炎性细胞因子检查。两组I/R后粘附血小板数均明显增加。与对照组相比，西维来司他组肝I/R后粘附血小板数量显著减少。此外，西维来司他改善了组织学结果的变化和肝酶的升高。但TNF-α、IL-1β和IL-6等炎性细胞因子无显著差异。血窦中的血小板粘附与I/R后肝功能障碍以及中性粒细胞相关。活化的中性粒细胞诱导血小板粘附在肝窦中。
Liver ischemia-reperfusion (I/R) injury is one of the most serious complications of hepatic surgery. In I/R, activated Kupffer cells cause platelet adhesion to sinusoidal endothelium as well as neutrophils and cause liver dysfunction. The aim of this study was to evaluate platelet dynamics in the hepatic microcirculation after I/R by intravital microscopy (IVM) and to clarify the relationship between platelet adhesion and neutrophil activation. Male Sprague-Dawley (SD) rats were divided into two groups: the control (administration of saline) group and the sivelestat group in which neutrophil activation was suppressed by sivelestat before I/R. The number of adherent platelets in sinusoid was observed up to 120 minutes after I/R by IVM. Samples of liver tissue and blood were taken for examination of histological findings, liver enzymes and inflammatory cytokines. The number of adherent platelets was significantly increased after I/R in both groups. Compared with the control group, the number of adherent platelets significantly decreased after hepatic I/R in the sivelestat group. Moreover, sivelestat improved changes of histological findings and elevation of liver enzymes. However, there was no significant difference in inflammatory cytokines of TNF-α, IL-1β or IL-6. Platelet adhesion in the sinusoid is associated with liver dysfunction after I/R as well as neutrophils. Activated neutrophils induce platelet adhesion in the sinusoid of the liver.