Identification of Mycoplasma and Other Microorganisms by Polyacrylamide-Gel Electrophoresis of Cell Proteins

Identification of Mycoplasma and Other Microorganisms by Polyacrylamide-Gel Electrophoresis of Cell Proteins
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通过细胞蛋白聚丙烯酰胺凝胶电泳鉴定支原体和其他微生物

DOI:
10.1128/jb.94.6.1807-1810.1967
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发表时间:
1967
影响因子:
3.2
通讯作者:
S. Rottem
S. Rottem
中科院分区:
生物学3区
文献类型:
--
作者:
S. Razin;S. Rottem

文献摘要

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不同物种支原体细胞的蛋白质在含有5 m尿素和35%醋酸的聚丙烯酰胺凝胶中产生高度可复制和物种特异性的电泳模式。这些电泳图谱可用于支原体的快速鉴定和分类。初步结果表明,该方法也可用于其他微生物的鉴定和分类。
The proteins of Mycoplasma cells of various species produce highly reproducible and species-specific electrophoretic patterns in polyacrylamide gels containing 5 m urea and 35% acetic acid. These electrophoretic patterns can be used for the rapid identification and classification of Mycoplasma. Preliminary results indicate that this method may also be used for the identification and classification of other microorganisms.