Vapor inhalation of cannabidiol (CBD) in rats
Vapor inhalation of cannabidiol (CBD) in rats
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DOI：
10.1016/j.pbb.2019.172741
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发表时间：
2019-09-01
影响因子：
3.6
通讯作者：
Taffe, Michael A.
中科院分区：
文献类型：
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作者：
Javadi-Paydar, Mehrak;Creehan, Kevin M.;Taffe, Michael A.
基本原理：大麻二酚（CBD）是一种在大麻属的许多菌株中发现的化合物，越来越多地用于电子烟液体以及其他产品。CBD的使用已被推广为许多声称的好处，尚未严格评估在preclinical studies.Objective：为了进一步验证吸入模型，以评估CBD在大鼠的影响。主要目标是确定蒸汽吸入后的血浆CBD水平，并将其与注射后观察到的水平进行比较。次要目标是确定是否在雄性Sprague-Dawley大鼠中产生低体温，以及CBD是否影响通过温水尾戒断测定的伤害感受。方法：从暴露于使用CBD（100，400 mg/mL，在丙二醇载体中）的电子烟装置产生的蒸汽30分钟的大鼠收集血液样品。单独的实验评估了CBD与尼古丁组合的体温反应（30 mg/mL）和对CBD的抗伤害性反应。CBD的蒸气吸入在雄性和雌性Wistar大鼠中产生浓度相关的血浆CBD水平，其在10或30 mg/kg CBD，i. p.产生的水平范围内。CBD在雄性Sprague-Dawley大鼠中产生剂量相关的低温，吸入尼古丁（30 mg/mL）可增强此作用。CBD吸入单独或与Delta（9）-tetrahydrocannabinol inhalation.Conclusions组合对抗伤害性感受没有影响：蒸汽吸入方法是一种合适的临床前模型，用于研究吸入CBD的影响。这种给药途径在大鼠中产生低体温，而在可比较的血浆CBD水平下，腹膜内注射不会。
Rationale: Cannabidiol (CBD), a compound found in many strains of the Cannabis genus, is increasingly available in e-cigarette liquids as well as other products. CBD use has been promoted for numerous purported benefits which have not been rigorously assessed in preclinical studies.Objective: To further validate an inhalation model to assess CBD effects in the rat. The primary goal was to determine plasma CBD levels after vapor inhalation and compare that with the levels observed after injection. Secondary goals were to determine if hypothermia is produced in male Sprague-Dawley rats and if CBD affects nociception measured by the warm water tail-withdrawal assay.Methods: Blood samples were collected from rats exposed for 30 min to vapor generated by an e-cigarette device using CBD (100, 400 mg/mL in the propylene glycol vehicle). Separate experiments assessed the body temperature response to CBD in combination with nicotine (30 mg/mL) and the anti-nociceptive response to CBD.Results: Vapor inhalation of CBD produced concentration-related plasma CBD levels in male and female Wistar rats that were within the range of levels produced by 10 or 30 mg/kg, CBD, i.p. Dose-related hypothermia was produced by CBD in male Sprague-Dawley rats, and nicotine (30 mg/mL) inhalation enhanced this effect. CBD inhalation had no effect on anti-nociception alone or in combination with Delta(9)-tetrahydrocannabinol inhalation.Conclusions: The vapor-inhalation approach is a suitable pre-clinical model for the investigation of the effects of inhaled CBD. This route of administration produces hypothermia in rats, while i.p. injection does not, at comparable plasma CBD levels.