CEREBRAL ENERGY-METABOLISM IN CYANIDE ENCEPHALOPATHY
CEREBRAL ENERGY-METABOLISM IN CYANIDE ENCEPHALOPATHY
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DOI：
10.1038/jcbfm.1989.23
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发表时间：
1989-04-01
影响因子：
6.3
通讯作者：
MACMILLAN, VH
中科院分区：
文献类型：
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作者：
MACMILLAN, VH
本研究记录了颈动脉内注射致死阈值量的KCN（2.5 mg. kg ~（-1）对大鼠脑能量代谢和组织学的影响。该剂量的KCN导致脑电图活动迅速消失，其基本上保持不存在长达3小时。脑代谢物测量0.25小时后KCN输注表明细胞色素氧化酶活性降低52%，乳酸增加600%，ATP减少32%，ADP增加73%，糖原减少85%。在随后的7天内对上述能量代谢物的测量显示，所有代谢物在6-24小时内恢复到控制状态。与能量代谢正常化相对应的是EEG和意识活动的恢复。氰化物暴露的动物的组织学检查显示，只有一只动物在0.5小时显示几个黑暗的神经元，两只动物在1小时轻微苍白的胼胝体和尾壳核，和一只动物在48小时与一个小的海马梗死的变化。结论是，它可能是不可能产生足够严重的干扰脑代谢与KCN注射，产生神经元损伤的纯粹的“组织毒性”机制。
This study documents the effects of an intracarotid artery injection of a lethal threshold amount of KCN (2.5 mg .cntdot. kg-1) on the energy metabolism and histology of the rat brain. This dose of KCN resulted in a rapid abolition of electroencephalographic activity, which remained essentially absent for up to 3 h. Cerebral metabolite measurements 0.25 h after KCN infusion indicated a 52% reduction in cytochrome oxidase activity, a 600% increase in lactate, a 32% reduction in ATP, a 73% increase in ADP, and an 85% decrease in glycogen. Measurements of the above energy metabolites over the ensuing 7 days showed a return to control of all metabolites by 6-24 h. Corresponding to the normalization of energy metabolism was a return of EEG and conscious activity. Histological examination of cyanide-expose animals revealed a paucity of change with only one animal at 0.5 h showing several dark neurons, two animals at 1 h with minor pallor of corus callosum and caudate-putamen, and one animal at 48 h with a small hippocampal infarction. It is concluded that it may be impossible to produce a serious enough disruption of cerebral metabolism with KCN injection, to produce neuronal damage by purely "histotoxic" mechanisms.