Production of recombinant proteins in tobacco guttation fluid
Production of recombinant proteins in tobacco guttation fluid
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DOI：
10.1104/pp.124.3.927
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发表时间：
2000-11-01
期刊：
影响因子：
7.4
通讯作者：
Raskin, I
中科院分区：
文献类型：
--
作者：
Komarnytsky, S;Borisjuk, NV;Raskin, I
枯食，即水分和溶解物质从未受伤的植物器官中流失，是高等植物的一种常见现象。我们利用内质网信号肽与重组蛋白序列融合，获得了转基因烟草（Nicotiana tabacum L. cv Wisconsin）植株，该植株通过叶片细胞间隙分泌三种不同遗传背景的异源蛋白（细菌木聚糖酶、水母绿色荧光蛋白和人胎盘碱性磷酸酶）进入烟草舌液。碱性磷酸酶的产率为每天1.1马克/克叶干重，该蛋白占灌胃液中总可溶性蛋白的近3%。注入液可以在整个工厂的提升过程中收集，从而为重组蛋白的生产提供了一个连续的、无损的系统。通过提高产量，取消提取，简化其下游加工，注入液具有提高重组蛋白生产技术效率的潜力。
Guttation, the loss of water and dissolved materials from uninjured plant organs, is a common phenomenon in higher plants. By using endoplasmic reticulum signal peptides fused to the recombinant protein sequences, we have generated transgenic tobacco (Nicotiana tabacum L. cv Wisconsin) plants that secrete three heterologous proteins of different genetic backgrounds (bacterial xylanase, green fluorescent protein of jellyfish [Aequorea victoria], and human placental alkaline phosphatase) through the leaf intercellular space into tobacco guttation fluid. Production rates of 1.1 mug/g of leaf dry weight per day were achieved fur alkaline phosphatase with this protein comprising almost 3% of total soluble protein in the guttation fluid. Guttation fluid can be collected throughout a plant's lift, thus providing a continuous and nondestructive system for recombinant protein production. Guttation fluid has the potential of increasing the efficiency of recombinant protein production technology by increasing yield, abolishing extraction, and simplifying its downstream processing.