Synergistic hepatoprotective effect of Schisandrae lignans with Astragalus polysaccharides on chronic liver injury in rats
Synergistic hepatoprotective effect of Schisandrae lignans with Astragalus polysaccharides on chronic liver injury in rats
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DOI：
10.1016/j.phymed.2009.02.004
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发表时间：
2009-09-01
期刊：
影响因子：
7.9
通讯作者：
Khalid, Rahman
中科院分区：
文献类型：
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作者：
Yan, Fei;Zhang, Qiao-Yan;Khalid, Rahman
本研究的目的是探讨五味子木脂素（LFS）与黄芪多糖（APS）对雄性SD大鼠慢性肝损伤的协同保肝作用。与对照组相比，每周两次皮下注射 10% CCl4，持续 3 个月，导致血清丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)、碱性磷酸酶 (ALP) 活性显着升高 (p < 0.001)。在肝脏中，给予 CCl4 后，观察到丙二醛 (MDA) 水平显着升高 (p < 0.001)，还原型谷胱甘肽 (GSH) (p < 0.05) 和过氧化氢酶 (CAT) (p < 0.001)、超氧化物歧化酶 (SOD) (p < 0.01) 水平降低。与 LFS 或 APS 给药相比，以“LFS (45mg/kg) + APS (150 mg/kg)”和“LFS (13·5 mg/kg) + APS (450 mg/kg)”剂量对大鼠进行“LFS+ASP”治疗显示出保肝和抗氧化作用，血清 ALT、AST、ALP 水平较低（p < 0.005 或 0.001）即可证明这一点与毒素治疗组相比，肝脏 MDA 浓度（p < 0.001）以及 SOD（p < 0.05 或 0.005）、CAT 活性（p < 0.01 或 0.005）、GSH 浓度（p < 0.05 或 0.005）更高。组织病理学检查显示毒素组出现严重的脂肪变性，而“LFS+APS”治疗组则观察到轻度损伤。上述参数的每种药物及其组合（单次治疗相同剂量）之间的药物相互作用系数（CDI）均小于1，表明LFS和APS表现出保肝和抗氧化特性，并在CCl4诱导的大鼠肝损伤中以协同方式发挥作用。 (c) 2009 年爱思唯尔有限公司。版权所有。
The aim of this study was to investigate the synergistic hepatoprotective effect of lignans from Fructus Schisandrae chinensis (LFS) with Astragalus polysaccharides (APS) on chronic liver injury in male Sprague-Dawley rats. Subcutaneous injection of 10% CCl4 twice a week for 3 months resulted in significantly (p < 0.001) elevated serum alanine aminotransferase (ALT), asparate aminotransferase (AST), alkaline phosphatase (ALP) activities compared to controls. In the liver, significantly elevated levels (p < 0.001) of malondialdehyde (MDA), lowered levels of reduced glutathione (GSH) (p < 0.05) and catalase (CAT) (p < 0.001), superoxide dismutase (SOD) (p < 0.01) were observed following CCl4 administration. 'LFS+ASP' treatment of rats at doses of 'LFS (45mg/kg) + APS (150 mg/kg)' and 'LFS (13 5 mg/kg) + APS (450 mg/kg)' displayed hepatoprotective and antioxidative effects than the administration of either LFS or APS, as evident by lower (p < 0.005 or 0.001) levels of serum ALT, AST, ALP and hepatic MDA (p < 0.001) concentration, as well as higher SOD (p < 0.05 or 0.005), CAT activities(p < 0.01 or 0.005), GSH concentration (p < 0.05 or 0.005) compared to the toxin treated group. Histopathological examinations revealed severe fatty degeneration in the toxin group, and mild damage in groups treated with 'LFS+APS' were observed. The coefficients drug interaction (CDI) between each individual drug and their combination (at the same dose of their single treatment) of these foregoing parameters were all less than 1, indicating that LFS and APS display hepatoprotective and antioxidant properties and act in a synergistic manner in CCl4 induced liver injury in rats. (c) 2009 Elsevier GmbH. All rights reserved.