INCREASED BASIC FIBROBLAST GROWTH-FACTOR MESSENGER-RNA FOLLOWING CONTUSIVE SPINAL-CORD INJURY
INCREASED BASIC FIBROBLAST GROWTH-FACTOR MESSENGER-RNA FOLLOWING CONTUSIVE SPINAL-CORD INJURY
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DOI：
10.1016/0169-328x(94)90026-4
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发表时间：
1994-03-01
期刊：
影响因子：
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通讯作者：
MOCCHETTI, I
中科院分区：
文献类型：
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作者：
FOLLESA, P;WRATHALL, JR;MOCCHETTI, I
神经营养因子似乎对损伤神经元的存活和潜在再生至关重要。周围神经系统的损伤导致许多神经营养分子的诱导。我们对中枢神经组织对损伤的反应知之甚少。我们检测了三种营养因子mRNA水平的变化，碱性和酸性成纤维细胞生长因子（bFGF, aFGF）和神经生长因子（NGF），在标准化的不完全性胸椎挫伤脊髓损伤（SCI）后。RNase保护实验显示，脊髓损伤后6小时，包括损伤部位的组织中bFGF mRNA的含量迅速增加（3倍）。颈椎或腰椎节段未见损伤的影响。损伤部位的bFGF mRNA在脊髓损伤后1天和7天仍显著升高。此外，在第7天，这种增加在解剖学上仅限于损伤部位的吻侧部分，这表明在维持高水平bFGF mRNA的过程中参与了特定的途径。脊髓损伤后aFGF mRNA水平未见变化。同样，在脊髓损伤后6小时、1天或7天，NGF及其高亲和受体（trkA） mRNA的表达也没有差异。我们对脊髓损伤对bFGF mRNA表达的特异性影响的观察支持了一种推测性假设，即bFGF可能在不完全性挫伤脊髓损伤后的部分功能恢复中发挥作用。
Neurotrophic factors appear to be crucial for the survival and potential regeneration of injured neurons. Injury of the peripheral nervous system results in the induction of a number of neurotrophic molecules. Less is known about the response of central nervous tissue to injury. We have examined changes in levels of mRNA for three trophic factors, basic and acidic fibroblast growth factor (bFGF, aFGF), and nerve growth factor (NGF), after a standardized incomplete thoracic contusive spinal cord injury (SCI). RNase protection assays showed a rapid increase (3-fold) in the content of bFGF mRNA by 6 hours after SCI in tissue that included the injury site. No effect of injury was seen in segments of cervical or lumbar cord. bFGF mRNA at the injury site remained significantly increased at 1 and 7 days after SCI. Further, at 7 days, the increase was anatomically restricted to the rostral portion of the injury site suggesting the involvement of specific pathways in the maintenance of high levels of bFGF mRNA. No change in the levels of aFGF mRNA was seen after SCI. Similarly, no difference in the expression of the mRNA for NGF or its high affinity receptor (trkA), were observed at 6 h, 1 or 7 days following SCI. Our observation of a specific effect of SCI on bFGF mRNA expression supports a speculative hypothesis that bFGF may play a role in the partial recovery of function seen following incomplete contusive spinal cord injury.