LncROR Promotes Bladder Cancer Cell Proliferation, Migration, and EpithelialMesenchymal Transition
LncROR Promotes Bladder Cancer Cell Proliferation, Migration, and EpithelialMesenchymal Transition
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DOI：
10.1159/000475910
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发表时间：
2017-01-01
影响因子：
--
通讯作者：
Huang, Jiefu
中科院分区：
文献类型：
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作者：
Chen, Yi;Peng, Ya;Huang, Jiefu
背景资料：LncRNA ROR是一种与多种人类癌症相关的肿瘤癌基因，已报道其通过靶向多个基因参与调节多种细胞过程，如增殖、凋亡和侵袭。然而，其在膀胱癌中的分子生物学功能尚未明确阐明。本研究旨在探讨ROR在膀胱癌中的表达水平及其功能。研究方法：通过qRT-PCR评估36对膀胱癌组织（和相应的非肿瘤组织）和膀胱癌细胞中的LncRNA ROR表达水平。采用MTT法、集落形成实验、流式细胞术、伤口愈合实验、细胞transwell实验、贴壁/脱离实验和Western blotting等方法观察ROR对BC细胞增殖、凋亡、迁移/侵袭和上皮间质转化（EMT）表型的影响。ZEB 1是ROR的目标。进行拯救测定以进一步证实ROR通过靶向ZEB 1促进BC细胞的进展。结果如下：LncRNA ROR在膀胱癌组织中上调（与邻近非肿瘤组织相比），并且在膀胱癌细胞中几乎过表达（与正常尿路上皮细胞系SVHUC-1细胞相比）。lncRNA ROR表达增加可明显促进肿瘤细胞增殖，抑制细胞凋亡，促进细胞转移，促进EMT表型的形成。ROR表达下调可明显抑制细胞增殖，促进细胞凋亡，抑制肿瘤转移，逆转EMT向MET转化。ZEB 1是ROR的靶基因，与癌组织中ROR水平呈正相关。结论：lncRNA ROR与膀胱癌细胞的肿瘤进展有关。(C)2017作者由S.发布Karger AG，巴塞尔
Background: LncRNA ROR, a tumor oncogene associated with various human cancers, has been reported to be involved in regulating various cellular processes, such as proliferation, apoptosis and invasion through targeting multiple genes. However, the molecular biological function in bladder cancer has not been clearly elucidated. The aim of this study is to explore ROR expression levels and evaluated its function in bladder cancer. Methods: LncRNA ROR expression levels in the 36 pairs of bladder cancer tissues (and corresponding non-tumor tissues) and bladder cancer cells were assessed by qRT-PCR. MTT assay, colony formation assay, flow cytometric analysis, wound healing assay, cell transwell assays, attachment/detachment and western blotting were performed to assess the effects of ROR on proliferation, apoptosis, migration/invasion and epithelial-to-mesenchymal (EMT) phenotypes in BC cells in vitro. ZEB1 is target of ROR. Rescue assays were performed to further confirm that ROR contributes to the progression of BC cells through targeting ZEB1. Results: LncRNA ROR was up-regulated in bladder cancer tissues (compared to adjacent non-tumor tissues) and was almost overexpression in bladder cancer cells (compared with normal urothelial cell line SVHUC-1 cells). Increased lncRNA ROR expression significantly promoted tumor cells proliferation, inhibited cells apoptosis, facilitated cells metastasis and contributed to the formation of EMT phenotype. While down-regulated ROR could obviously inhibit cells proliferation, promote cells apoptosis, inhibit metastasis and reverse EMT to MET. ZEB1 was a target gene of ROR and was positive correlation with the level of ROR in cancer tissues. Conclusion: These results indicated that lncRNA ROR was associated with tumor progression in bladder cancer cells. (C) 2017 The Author(s) Published by S. Karger AG, Basel