WHEAT CULTIVAR IDENTIFICATION BY GLIADIN ELECTROPHOREGRAMS .1. APPARATUS, METHOD AND NOMENCLATURE
WHEAT CULTIVAR IDENTIFICATION BY GLIADIN ELECTROPHOREGRAMS .1. APPARATUS, METHOD AND NOMENCLATURE
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DOI：
10.4141/cjps78-076
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发表时间：
1978-01-01
影响因子：
1.2
通讯作者：
ZILLMAN, RR
中科院分区：
文献类型：
--
作者：
BUSHUK, W;ZILLMAN, RR
介绍了一种用于麦胶蛋白聚丙烯酰胺凝胶电泳的装置和方法。由于麦胶蛋白模式（电泳图）是一种基因型特征，电泳为小麦品种鉴定提供了一种很有前途的方法。电泳图提出了一些小麦品种，以说明可以通过所描述的方法获得的结果。本文还提出了用这种方法分离的麦胶蛋白带（组分）的新命名法。命名法使用加拿大硬红春小麦品种侯爵的电泳图中的主要波段作为参考。它被赋予一个任意的0.50的流动性；在相同的电泳条件下，“侯爵”和其他品种的所有其他条带的电泳迁移率相对于参考带的0.50进行了鉴定。
An apparatus and method are described for polyacrylamide gel electrophoresis of gliadins. Since the gliadin pattern (electrophoregram) is a genotypic character, electrophoresis offers a promising means for identifying wheat cultivars. Electrophoregrams are presented for a number of wheat cultivars to illustrate the results that can be obtained by the method described. A new nomenclature for gliadin bands (components) separated by this method is also presented. The nomenclature uses a major band in the electrophoregram of the Canadian hard red spring wheat cultivar Marquis as the reference. It was assigned an arbitrary mobility of 0.50; all other bands of ''Marquis'' and other cultivars are identified on the basis of electrophoretic mobility relative to 0.50 for the reference band under identical electrophoresis conditions.