3D visualization of HIV virions by cryoelectron tomography.
3D visualization of HIV virions by cryoelectron tomography.
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DOI：
10.1016/s0076-6879(10)83014-9
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发表时间：
2010
影响因子：
--
通讯作者：
Winkler, Hanspeter
中科院分区：
文献类型：
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作者：
Liu, Jun;Wright, Elizabeth R.;Winkler, Hanspeter
The structure of the human immunodeficiency virus (HIV) and some of its components have been difficult to study in three-dimensions (3D) primarily because of their intrinsic structural variability. Recent advances in cryoelectron tomography (cryo-ET) have provided a new approach for determining the 3D structures of the intact virus, the HIV capsid, and the envelope glycoproteins located on the viral surface. A number of cryo-ET procedures related to specimen preservation, data collection, and image processing are presented in this chapter. The techniques described herein are well suited for determining the ultrastructure of bacterial and viral pathogens and their associated molecular machines in situ at nanometer resolution.
