Global small-angle X-ray scattering data analysis for multilamellar vesicles: the evolution of the scattering density profile model.
Global small-angle X-ray scattering data analysis for multilamellar vesicles: the evolution of the scattering density profile model.
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DOI：
10.1107/s1600576713029798
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发表时间：
2014-02-01
影响因子：
6.1
通讯作者：
Pabst G
中科院分区：
文献类型：
--
作者：
Heftberger P;Kollmitzer B;Heberle FA;Pan J;Rappolt M;Amenitsch H;Kučerka N;Katsaras J;Pabst G
高分辨率散射长度密度分析方法已从单层脂质囊泡推广到多层脂质囊泡。该方法可以应用于X射线数据，或X射线和中子数据的联合分析，产生改进的细化的脂质骨架的位置。非常成功的散射密度分布（SDP）模型，用于联合分析小角度X射线和中子散射数据从单层囊泡，已被改编为使用的数据从完全水合，液晶多层囊泡（MLV）。使用遗传算法，这种新方法能够提供高分辨率的结构信息，以及确定双层弹性弯曲波动从独立的X射线数据。结构参数，如双层厚度和面积每脂质测定了一系列的饱和和不饱和脂质，以及二元混合物与胆固醇。结果是在良好的协议与以前报道的SDP数据，其中使用中子和X射线数据。在分析中纳入氘代和非氘代MLV中子数据改善了脂质骨架信息，但在实验误差范围内并未改善关于双层厚度和每种脂质面积的结构数据。
The high-resolution scattering length density analysis method has been generalized from unilamellar to multilamellar lipid vesicles. The method may be applied to X-ray data only, or to a joint analysis of X-ray and neutron data, yielding an improved refinement of the lipid backbone position. The highly successful scattering density profile (SDP) model, used to jointly analyze small-angle X-ray and neutron scattering data from unilamellar vesicles, has been adapted for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). Using a genetic algorithm, this new method is capable of providing high-resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone X-ray data. Structural parameters such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. The results are in good agreement with previously reported SDP data, which used both neutron and X-ray data. The inclusion of deuterated and non-deuterated MLV neutron data in the analysis improved the lipid backbone information but did not improve, within experimental error, the structural data regarding bilayer thickness and area per lipid.