Usefulness of human coagulation and fibrinolysis assays in domestic pigs.
Usefulness of human coagulation and fibrinolysis assays in domestic pigs.
复制标题
人类凝血和纤溶测定在家猪中的有用性。
作者：
A. Münster;A. Olsen;E. Bladbjerg
猪是凝血和纤溶研究中常用的动物模型。因此，在该领域内应用的测定的有用性以及参考区间的知识是至关重要的。在本文报告的研究中，我们研究了市售人凝血和纤溶试验用于猪血浆的适用性。总共对从家猪中获得的血浆进行了22项功能和免疫测定，并测量了以下凝血和纤溶变量：凝血酶原时间、活化部分凝血活酶时间、组织因子、组织因子途径抑制剂、因子VII、蛋白C、蛋白S、凝血酶原片段1+2、抗凝血酶、凝血酶-抗凝血酶复合物、纤维蛋白原、可溶性纤维蛋白，尿激酶型纤溶酶原激活物、纤溶酶抑制剂、纤溶酶原激活物抑制剂1和D-二聚体。我们发现，12个功能测定中有11个，但10个免疫测定中只有3个适用于猪血浆，我们确定了这些变量的正常范围。我们的结论是，人类功能测定是有用的猪血浆，而只有少数免疫测定可以使用。然而，在结果解释和向人体结果外推时必须采取预防措施，因为猪和人体值之间的可能差异可能是由于种属变异和/或方法学错误所致。
Pigs are often used as animal models in research on blood coagulation and fibrinolysis. The usefulness of the assays applied within this field, and the knowledge of reference intervals are therefore essential and of utmost importance. In the study reported here, we investigated the applicability of commercial human coagulation and fibrinolysis assays for use with porcine plasma. In total, 22 functional and immunologic assays were applied to plasma obtained from domestic pigs, and the following blood coagulation and fibrinolysis variables were measured: prothrombin time, activated partial thromboplastin time, tissue factor, tissue factor pathway inhibitor, factor VII, protein C, protein S, prothrombin fragment 1+2, antithrombin, thrombin-antithrombin complexes, fibrinogen, soluble fibrin, urokinase-type plasminogen activator, plasmin inhibitor, plasminogen activator inhibitor 1, and D-dimer. We found that 11 of 12 functional assays, but only 3 of 10 immunoassays, were applicable to porcine plasma, and we determined the normal range of these variables. We conclude that human functional assays are useful in porcine plasma, whereas only a few immunologic assays can be used. However, precautions must be taken in interpretation of the results and in extrapolation toward human results because possible differences between porcine and human values can be due to species variations and/or methodologic errors.