Mitochondrial DNA phylogeography of host races of the goldenrod ball gallmaker, Eurosta solidaginis (Diptera: Tephritidae)
Mitochondrial DNA phylogeography of host races of the goldenrod ball gallmaker, Eurosta solidaginis (Diptera: Tephritidae)
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DOI：
10.2307/2410850
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发表时间：
1996-04-01
期刊：
影响因子：
3.3
通讯作者：
Way, PA
中科院分区：
文献类型：
--
作者：
Brown, JM;Abrahamson, WG;Way, PA
我们确定了系统发育关系和线粒体单倍型的地理分布的两个主机种族的实腹实蝇，gallmaker攻击种黄花（一枝黄花）。我们对来自8个S.的单个个体的线粒体细胞色素氧化酶I和II亚基的3'端492 bp进行了测序。绿茶-和10 S。altissima相关的人口在其范围内在北美东部和两个外群物种，Eurosta逗号（两个人口）和E。筛状的单倍型分为两组（“E”和“W "分支），这是由四个取代，其中一个发生在识别位点的DdeI限制酶不同。我们使用限制性位点的存在或不存在来调查总共11个S。茶（20个个体）和20个S. altissima（43个个体）寄主种群。无论地理来源，所有的茶蝇单倍型进行E-进化枝单倍型，而altissima-fly单倍型的地理分区。密歇根州东部的高脚蝇为单倍型E，而密歇根州西部的高脚蝇为单倍型W，在密歇根州南部发现了混合种群。这些模式证实了早期的等位酶调查表明，S。altissima是gallmaker的祖先宿主，但也表明，美洲斑腿蝇种群来自美国东部的altissima蝇种群。这些数据支持的结论，行为和生态学研究表明，转移到衍生主机是由逃避天敌。
We determined the phylogenetic relationships and geographic distribution of mitochondrial haplotypes of two host races of the tephritid fly Eurosta solidaginis, a gallmaker that attacks species of goldenrod (Solidago). We performed a preliminary survey by sequencing 492 bp from the 3' ends of the mitochondrial cytochrome oxidase I and II subunits from a single individual from eight S. gigantea- and 10 S. altissima-associated populations across their range in eastern North America and from two outgroup species, Eurosta comma (two populations) and E. cribrata. Eurosta solidaginis haplotypes fell into two groups (''E'' and ''W'' clades), which differed by four substitutions, one of which occurred within the recognition site of the DdeI restriction enzyme. We used the presence or absence of the restriction site to survey a total of 11 S. gigantea (20 individuals) and 20 S. altissima (43 individuals) host-race populations. All gigantea-fly haplotypes regardless of geographic origin carried the E-clade haplotype, whereas altissima-fly haplotypes were geographically partitioned. Altissima flies east of Michigan were of haplotype E, whereas those west of Michigan were of haplotype W, with mixed populations found in lower Michigan. These patterns confirm an earlier allozyme survey that suggested that S. altissima is the ancestral host for the gallmaker, but also suggest that the gigantea fly populations were derived from eastern U.S. altissima fly populations. The data support the conclusions of behavioral and ecological studies indicating that the shift to the derived host was facilitated by escape from natural enemies.