TYPE-VII COLLAGEN FORMS AN EXTENDED NETWORK OF ANCHORING FIBRILS
TYPE-VII COLLAGEN FORMS AN EXTENDED NETWORK OF ANCHORING FIBRILS
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DOI：
10.1083/jcb.104.3.611
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发表时间：
1987-03-01
影响因子：
7.8
通讯作者：
BURGESON, RE
中科院分区：
文献类型：
--
作者：
KEENE, DR;SAKAI, LY;BURGESON, RE
VII型胶原蛋白是胶原蛋白家族的新成员之一。包括超微结构免疫定位在内的各种证据此前已表明，VII型胶原蛋白是锚定原纤维的主要结构成分，紧邻许多上皮细胞的致密层下方发现。在本研究中，超微结构免疫定位与单克隆抗体和单特异性多克隆抗体的VII型胶原蛋白和单克隆抗体的IV型胶原蛋白表明，无定形电子致密结构，我们称之为“锚定斑块”是皮肤和角膜的基底膜区的正常功能。这些斑块含有IV型胶原和VII型胶原的羧基末端结构域。带状锚定纤维从致密层和这些斑块延伸，并且可以看到将斑块与致密层和其他锚定斑块桥接。这些观察结果导致锚定纤维和锚定斑块的多层网络的假设，其位于几个含锚定纤维的组织的基底层之下。这种延伸的网络能够包埋大量带状胶原原纤维、微纤维和其他基质基质组分。这些观察结果支持了锚定纤维提供致密层与其下层基质的额外粘附的假设。
Type VII collagen is one of the newly identified members of the collagen family. A variety of evidence, including ultrastructrual immunolocalization, has previously shown that type VII collagen is a major structural component of anchoring fibrils, found immediately beneath the lamina densa of many epithelia. In the present study, ultrastructural immunolocalization with monoclonal and monospecific polyclonal antibodies to type VII collagen and with a monoclonal antibody to type IV collagen indicates that amorphous electron-dense structures which we termed "anchoring plaques" are normal features of the basement membrane zone of skin and cornea. These plaques contain type IV collagen and the carboxyl-terminal domain of type VII collagen. Banded anchoring fibrils extend from both the lamina densa and from these plaques, and can be seen bridging the plaques with the lamina densa and with other anchoring plaques. These observations lead to the postulation of a multilayered network of anchoring fibrils and anchoring plaques which underlies the basal lamina of several anchoring fibril-containing tissues. This extended network is capable of entrapping a large number of banded collagen fibrils, microfibrils, and other stromal matrix components. These observations support the hypothesis that anchoring fibrils provide additional adhesion of the lamina densa to its underlying stroma.