Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa
Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa
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DOI：
10.1101/gr.8.1.18
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发表时间：
1998-01-01
期刊：
影响因子：
7
通讯作者：
Sibley, LD
中科院分区：
文献类型：
--
作者：
Ajioka, JW;Boothroyd, JC;Sibley, LD
为了加速弓形虫基因的发现和基因定位，我们从随机选择的速殖子cDNA的5'端产生了超过7000个新的EST。将EST与现有基因数据库进行比较，确定了500多个新T.通过与保守蛋白质家族共享的序列基序，包括参与转录、翻译、蛋白质分泌、信号转导、细胞骨架组织和代谢的因子，弓形虫基因被克隆。尽管在识别新基因方面取得了成功，但超过50%的EST对应于未知功能的基因，反映了这种寄生虫的不同进化状态。一个新认识的基因类是基于其相似性的序列只知道从其他成员的同一门，因此确定的序列，显然是有限的顶复门。这些基因可能是这组医学上重要的寄生虫共同途径的基础，因此确定了干预的潜在目标。
To accelerate gene discovery and Facilitate genetic mapping in the protozoan parasite Toxoplasma gondii, we have generated >7000 new ESTs From the 5' ends of randomly selected tachyzoite cDNAs. Comparison of the ESTs with the existing gene databases identified possible functions for more than 500 new T. gondii genes by virtue of sequence motifs shared with conserved protein families, including factors involved in transcription, translation, protein secretion, signal transduction, cytoskeleton organization, and metabolism. Despite this success in identifying new genes, more than 50% of the ESTs correspond to genes of unknown function, reflecting the divergent evolutionary status of this parasite. A newly recognized class of genes was identified based on its similarity to sequences known only from other members of the same phylum, therefore identifying sequences that are apparently restricted to the Apicomplexa. Such genes may underlie pathways common to this group of medically important parasites, therefore identifying potential targets For intervention.