Targeting of EGF-displayed protein nanoparticles with anticancer drugs
Targeting of EGF-displayed protein nanoparticles with anticancer drugs
复制标题
DOI：
10.1002/jbm.b.33162
复制
发表时间：
2014-11-01
影响因子：
3.4
通讯作者：
Kobatake, Eiry
中科院分区：
文献类型：
--
作者：
Matsumoto, Rie;Nara, Rieko;Kobatake, Eiry
基于蛋白质的药物载体的发展已经得到了很好的研究。我们之前构建了一种基于蛋白质的纳米颗粒，由基因工程的弹性蛋白样多肽(ELP)和融合的聚天冬氨酸尾巴(ELPD)组成。自组装的ELPD纳米粒子的大小受聚天冬氨酸链的带电排斥作用的调节。在本研究中，将表皮生长因子(EGF)基因融合到ELPD的C末端，以赋予ELPD纳米颗粒主动的靶向能力。我们考察了含有EGF的纳米颗粒的形成及其靶向能力。发现含有融合EGF的ELPD形成了纳米颗粒，在其表面显示了多价的EGF。携带抗癌药物紫杉醇的EGF展示纳米颗粒被内化到过度表达EGF受体的细胞中，并诱导细胞死亡。(C)2014年威利期刊公司。
The development of protein-based carriers for drug delivery has been well studied. We previously constructed a protein-based nanoparticle consisting of genetically engineered elastin-like polypeptides (ELPs) with a fused polyaspartic acid tail (ELPD). The size of the self-assembled ELPD nanoparticles was regulated by charged repulsion of the poly-aspartic acid chains. In the present study, epidermal growth factor (EGF) was genetically fused to the C-terminus of ELPD to impart an active targeting ability to the ELPD nanoparticles. We examined the nanoparticle formation with EGF as well as its targeting ability. ELPD with fused EGF was found to form nanoparticles that displayed multivalent EGFs on their surface. EGF-displayed nanoparticles loaded with the anti-cancer drug paclitaxel were internalized into cells overexpressing the EGF receptor, and induced cell death. (C) 2014 Wiley Periodicals, Inc.