Increased bone marrow angiogenesis in B cell chronic lymphocytic leukemia
Increased bone marrow angiogenesis in B cell chronic lymphocytic leukemia
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DOI：
10.1038/sj.leu.2401825
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发表时间：
2000-08-01
期刊：
影响因子：
11.4
通讯作者：
Peterson, LC
中科院分区：
文献类型：
--
作者：
Kini, AR;Kay, NE;Peterson, LC
最近的研究表明，除了在实体肿瘤的生长和转移中所扮演的角色外，血管生成可能参与了血液系统恶性肿瘤的发病机制。本研究通过对12例B细胞慢性淋巴细胞白血病(B-CLL)骨髓环钻活检组织切片的微血管密度和热点密度的测量，对B细胞慢性淋巴细胞白血病(B-CLL)的血管生成程度进行了定量，并与正常骨髓组织切片(n=11)进行了比较。B-CLL的平均微血管计数/高倍视野为7.64，而对照组的平均微血管计数/高倍视野为2.11(P=0.0001)。B-CLL切片的平均热点密度(14.83个/热点)也显著高于对照骨髓切片的平均热点密度(7.09个/热点)(P=0.0008)。微血管密度和热点密度均与B-CLL的临床分期呈正相关。在另一组B-CLL患者中，尿中血管生成肽碱性成纤维细胞生长因子水平(2216.5 pg/g，n=14)显著高于正常对照组(1084pg/g，n=58)(P=0.0001)。提示血管生成可能参与了B-CLL的发病机制。
Recent studies have shown that angiogenesis may be involved in the pathogenesis of hematopoietic malignancies, apart from its well-characterized role in the growth and metastasis of solid tumors. In this study, we quantified the degree of angiogenesis in B cell chronic lymphocytic leukemia (B-CLL) by measuring the microvessel density and hotspot density in bone marrow trephine biopsy sections with B-CLL involvement (n = 12) and compared it to normal bone marrow sections (n = 11). The B-CLL samples had a mean microvessel count/high power field (hpf) of 7.64 while the control samples had a mean microvessel count/hpf of 2.11 (P = 0.0001). The mean hotspot density in the B-CLL sections (14.83/hotspot) was also significantly higher (P = 0.0008) than the mean hotspot density in control bone marrow sections (7.09/hotspot). Both the microvessel density and hotspot density correlated positively with the clinical stage of the B-CLL patients. In a separate cohort of B-CLL patients, the median urine level of the angiogenic peptide, basic fibroblast growth factor (2216.5 pg/g, n = 14), was significantly higher (P = 0.0001) than the bFGF level in normal controls (1084 pg/g, n = 58). These results indicate that angiogenesis may be involved in the pathogenesis of B-CLL.