Microencapsulation of bovine spermatozoa.
Microencapsulation of bovine spermatozoa.
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牛精子的微囊化。
DOI：
10.2527/jas1985.6061631x
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发表时间：
1985
影响因子：
3.3
通讯作者：
F. Lim
中科院分区：
文献类型：
--
作者：
R. Nebel;J. Bame;R. G. Saacke;F. Lim
进行了两个实验来检验牛精子微囊化用于人工授精的功效。在Exp。如图 1 所示，精子以三种不同的浓度（45、90 和 180 X 10(6) 精子/ml）封装在 0.75 毫米或 1.5 毫米（直径）的微胶囊中，并在 37℃ 下体外培养 24 小时。每个浓度的未封装样品作为对照。在孵育 2、12 和 24 小时时评估胶囊内容物的精子活力百分比和完整顶体。孵育24小时后评估胶囊的脆性。精子的活力不受精子浓度或胶囊大小的影响，与对照组相比，胶囊后的细胞损伤不明显。胶囊的脆性不受胶囊大小的影响；然而，随着精子浓度的增加，胶囊的完整性降低（P 小于 0.05）。在Exp。 2、使用冻融精液，测量蛋黄含量、甘油的存在和精子活力对微囊化成功的影响。增量剂是 2.9% 柠檬酸钠和甘油 (7% v/v) 以及 0、5、10 或 15% 蛋黄 (v/v)。主观评价胶囊尺寸和形状的均匀性。胶囊完整性和均匀性不受甘油、精子活力或蛋黄水平高达 10% v/v 的影响；然而，将精子封装在 15% 卵黄缓冲液中增加了胶囊大小和形状的异质性。含有 10% 或 15% 卵黄 v/v 的扩展剂时，封装精子的活力最高。 5% 蛋黄稀释剂的活力降低是由于与冷冻相关的预封装损伤。微囊化程序与精子活力兼容，并且可以适应人工授精中使用的可接受的增量系统。
Two experiments were conducted to examine the efficacy of microencapsulation of bovine spermatozoa for use in artificial insemination. In Exp. 1, sperm were encapsulated at three different concentrations (45, 90 and 180 X 10(6) sperm/ml) in either .75- or 1.5-mm (diameter) microcapsules and incubated in vitro for 24 h at 37 C. Unencapsulated samples of each concentration served as controls. Capsule contents were evaluated for percentage of sperm motility and intact acrosomes at 2, 12 and 24 h of incubation. Capsule fragility was evaluated after 24 h incubation. Viability of spermatozoa was not influenced by sperm concentration or capsule size, and compared with controls, cellular injury after encapsulation was not apparent. Fragility of capsules was unaffected by capsule size; however, as the sperm concentration increased, integrity of the capsules decreased (P less than .05). In Exp. 2, using frozen-thawed semen, the effect of egg yolk content, presence of glycerol and viability of spermatozoa on the success of microencapsulation was measured. The extender was 2.9% sodium citrate with glycerol (7% v/v) and either 0, 5, 10 or 15% egg yolk (v/v). Uniformity of capsules in size and shape was evaluated subjectively. Capsule integrity and uniformity were unaffected by glycerol, sperm viability or egg yolk level up to 10% v/v; however, encapsulation of spermatozoa in 15%-yolk buffer increased the heterogeneity in capsule size and shape. Viability of encapsulated spermatozoa was maximal for extenders containing 10 or 15% yolk v/v. Reduced viability for the 5% yolk extender was due to pre-encapsulation injury associated with freezing. Microencapsulation procedures are compatible with sperm viability and can be adapted to an acceptable extender system used in artificial insemination.