The discrimination of high-risk HPV types by in situ hybridization and the polymerase chain reaction
The discrimination of high-risk HPV types by in situ hybridization and the polymerase chain reaction
复制标题
原位杂交和聚合酶链反应鉴别高危型HPV
DOI：
10.1007/bf00163814
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
J. McGee
中科院分区：
文献类型：
--
作者：
C. Herrington;S. Anderson;A. Graham;J. McGee
SummaryThe parameter Tmt has been defined by non-isotopic in situ hybridization and describes the tissue melting temperature (Tmt) of human papillomavirus (HPV) DNA sequences. In this study, multiple in situ hybridization signals for HPV types 16, 31 and 33 in individual archival biopsies hybridized with genomic probes are shown by polymerase chain reactions to be due to cross-hybridization of probe sequences to a single tissue target. Tmt is independent of viral type but depends on the homology between probe and target when using nick-translated whole genomic probes. The difference between Tm and Tmt is not due to the presence of viral capsid protein. Multiple HPV signals in archival material should not therefore be interpreted as indicative of multiple HPV infection unless adequate stringency conditions have been employed or they are present in morphologically distinct areas of the biopsy.Furthermore, extrapolation of calculated DNA homologies to non-isotopic in situ hybridization analysis may not be appropriate. A hybridization signal does not imply probe and target identity: this has implications for HPV typing in clinical material.
