Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
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免疫系统中的独特型连接。
DOI：
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发表时间：
1983
影响因子：
15.3
通讯作者：
G. Lewis
中科院分区：
文献类型：
--
作者：
P. Hornbeck;G. Lewis
描述了一种新的交叉反应独特表位家族（CRIAD 8），其含有结合不同表位的抗体亚群。一个亚群天然存在于来自品系A小鼠的正常血清中，主要存在于IgG 2和IgG 3亚类上，不结合对偶氮苯胂酸盐（阿坝）+，不表达CRI 5Ci，并且可以被低剂量的抗独特型抗体（AD 8）选择性地刺激。第二亚群在正常血清中未发现，结合阿坝，在所有IgG亚类上发现，表达CRI 5Ci，并且被ABA缀合的蛋白质选择性刺激。由于在抗体的两个亚群上都发现了CRIAD 8，并且由于每个亚群可以选择性扩增，因此可以研究阿坝8+组的扩增对阿坝+CRIAD 8+组中ABA-匙孔血蓝蛋白（KLH）引起的后续应答的影响。在这些实验中，先前用AD 8免疫限制了阿坝+CRIAD 8+组对ABA-KLH的后续应答。此外，只有刺激ABA-CRIAD 8+组的那些剂量的AD 8降低了阿坝+CRIAD 8+组对ABA-KLH的响应性，表明这两种现象是因果相关的。这些发现表明CRIAD 8与调控独特位相关性很好，并且具有不同抗原结合特异性的淋巴细胞克隆的免疫应答可以相互影响，因为它们共享这样的独特位。这些结果强烈支持免疫系统的网络组织。
A new cross-reactive idiotope family (CRIAD8) is described that contains subpopulations of antibodies binding to different epitopes. One subpopulation occurs naturally in normal sera from strain A mice, is found mainly on IgG2 and IgG3 subclasses, does not bind p- azobenzenearsonate (ABA)+, does not express CRI5Ci, and can be selectively stimulated by low doses of antiidiotype antibody (AD8). The second subpopulation is not found in normal serum, binds ABA, is found on all IgG subclasses, expresses CRI5Ci, and is selectively stimulated by ABA-conjugated proteins. Since CRIAD8 was found on both subpopulations of antibody, and since each subpopulation could be selectively expanded, it was possible to study the effect that expansion of the ABA- CRIAD8+ set had on subsequent responses elicited by ABA-keyhole limpet hemocyanin (KLH) in the ABA+ CRIAD8+ set. In these experiments, prior immunization with AD8 restricted the subsequent response of the ABA+ CRIAD8+ set to ABA-KLH. Furthermore, only those doses of AD8 that stimulated the ABA-CRIAD8+ set reduced the responsiveness of the ABA+ CRIAD8+ set to ABA-KLH, suggesting that the two phenomena are causally related. These findings argue that CRIAD8 correlates well with a regulatory idiotope and that immune responses by lymphocyte clones that have different antigen-binding specificities can affect one another as a result of their sharing such an idiotope. These results strongly favor a network organization of the immune system.
具有共同独特型的苯胂酸基团抗体的结合特异性的异质性程度。
影响因子：
3.6
作者：
Kresina,TF;Rosen,SM;Nisonoff,A
通讯作者：
Nisonoff,A