BCL-X IS EXPRESSED IN EMBRYONIC AND POSTNATAL NEURAL TISSUES AND FUNCTIONS TO PREVENT NEURONAL CELL-DEATH
BCL-X IS EXPRESSED IN EMBRYONIC AND POSTNATAL NEURAL TISSUES AND FUNCTIONS TO PREVENT NEURONAL CELL-DEATH
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DOI：
10.1073/pnas.92.10.4304
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
NUNEZ, G
中科院分区：
文献类型：
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作者：
GONZALEZGARCIA, M;GARCIA, I;NUNEZ, G
以前的研究表明bcl-2原癌基因是神经元存活的潜在调节因子，然而，缺乏功能性bcl-2的小鼠表现出正常的中枢神经系统（CNS）发育和维持。由于bcl-2似乎对神经元存活不利，我们检测了bcl-2死亡调节基因家族的另一个成员bcl-x的表达和功能，Bcl-2在胚胎发育期间在神经元组织中表达，但在成年CNS中下调。与此相反，Bcl-x（L）在成年中枢神经系统的神经元中仍有表达。两种不同形式的bcl-x mRNA及其相应产物Bcl-x（L）和Bcl-x（β）在中枢神经系统的胚胎和成年神经元中均有表达。将bcl-x（L）和bcl-x（β）cDNA显微注射到初级交感神经元中可抑制神经生长因子戒断诱导的神经元死亡。Bcl-x蛋白似乎在成人CNS神经元存活的调节中起重要作用。
Previous studies have implicated the bcl-2 protooncogene as a potential regulator of neuronal survival, However, mice lacking functional bcl-2 exhibited normal development and maintenance of the central nervous system (CNS), Since bcl-2 appears dispensable for neuronal survival, we have examined the expression and function of bcl-x, another member of the bcl-2 family of death regulatory genes, Bcl-2 is expressed in neuronal tissues during embryonic development but is down-regulated in the adult CNS. In contrast, Bcl-x(L) expression is retained in neurons of the adult CNS, Two different forms of bcl-x mRNA and their corresponding products, Bcl-x(L) and Bcl-x(beta), were expressed in embryonic and adult neurons of the CNS, Microinjection of bcl-x(L) and bcl-x(beta) cDNAs into primary sympathetic neurons inhibited their death induced by nerve growth factor withdrawal, Thus, Bcl-x proteins appear to play an important role in the regulation of neuronal survival in the adult CNS.