α-endosulfine, a potential regulator of insulin secretion, is required for adult tissue growth control in Drosophila
α-endosulfine, a potential regulator of insulin secretion, is required for adult tissue growth control in Drosophila
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DOI：
10.1016/j.ydbio.2003.10.028
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发表时间：
2004-02-15
影响因子：
2.7
通讯作者：
Spradling, AC
中科院分区：
文献类型：
--
作者：
Drummond-Barbosa, D;Spradling, AC
α-内硫醚是一种小蛋白，被认为可以调节离子通道活性和胰岛素分泌，但体内研究一直很缺乏。我们已经建立了果蝇卵巢作为研究成体组织生长调节的模型系统，并证明了胰岛素途径在卵巢细胞对营养变化的增殖反应中的作用。在这里，我们发现果蝇α-内硫精(Dendos)基因也是这种反应所必需的，该基因的蛋白在卵巢的生殖系和体细胞中表达，也在脑和肠道的某些区域表达。这一要求是非细胞自主的，这与树突在分泌果蝇胰岛素样肽(DILPs)中的作用一致，DILPs是对营养变化的增殖反应所必需的。我们的结果表明，Dendos在卵子发生中也是一个独特的过程所必需的，即对14期卵母细胞的渗透调节，并且这一要求是细胞自主的，这与哺乳动物同源物的研究表明的在离子通道调节中的作用是一致的。(C)2003 Elsevier Inc.保留所有权利。
alpha-Endosulfine is a small protein that has been proposed to regulate ion channel activity and insulin secretion, but in vivo studies have been lacking. We have previously established the Drosophila ovary as a model system in which to study adult tissue growth regulation, and demonstrated a role of the insulin pathway in the proliferative response of ovarian cells to nutritional changes. Here, we find that the Drosophila alpha-endosulfine (dendos) gene, whose protein is expressed in germline and somatic cells of the ovary, as well as in the brain and certain regions of the intestine, is also required for this response. This requirement is non-cell autonomous, which is consistent with a role of dendos in secretion of Drosophila insulin-like peptides (DILPs), required for the proliferative response to nutritional changes. Our results show that dendos is also required for a distinct process in oogenesis, namely, the osmotic regulation of stage 14 oocytes, and that this requirement is cell autonomous, consistent with the role in ion channel regulation suggested by studies of the mammalian homologues. (C) 2003 Elsevier Inc. All rights reserved.