The mitochondrial genome of the moss Physcomitrella patens sheds new light on mitochondrial evolution in land plants
The mitochondrial genome of the moss Physcomitrella patens sheds new light on mitochondrial evolution in land plants
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DOI：
10.1093/molbev/msl198
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发表时间：
2007-03-01
影响因子：
10.7
通讯作者：
Sato, Naoki
中科院分区：
文献类型：
--
作者：
Terasawa, Kimihiro;Odahara, Masaki;Sato, Naoki
研究轮藻和苔藓植物的系统发育位置及其基因组特征对了解早期陆生植物的进化具有重要意义。在这里，我们报告了完整的核苷酸序列（105，340 bp）的环形映射线粒体DNA的苔藓小立碗藓。现有的证据表明，在开花植物的线粒体基因组的多部分结构不发生在立碗藓。它含有3种rRNA（rn1、rns和rrn5）、24种tRNA和42种保守线粒体蛋白（14种核糖体蛋白、4种ccm蛋白、9种烟酰胺腺嘌呤二核苷酸脱氢酶亚基、5种ATP酶亚基、2种琥珀酸脱氢酶亚基、脱辅基细胞色素B、3种细胞色素氧化酶亚基和4种其他蛋白）的基因。我们估计有5个tRNA基因可能是由核基因组编码的。在立碗藓属、轮藻属、球毛球壳藻属和地钱属中，线粒体基因组的整体结构是相似的，具有容易识别的倒位和易位。与被子植物和绿藻线粒体基因组的显着共线性没有检测到。18个保守蛋白的系统发育分析表明，苔藓苔类分支是姐妹被子植物，这是与以前的叶绿体基因的分析，但不符合一些分析使用线粒体序列。在立碗藓属中，16个基因中存在27个内含子。它的9个内含子位置与被子植物共有，4个与地钱属共有，而地钱属与被子植物只有一个内含子位置。系统发育分析以及同线结构表明立碗藓属和地钱属植物的线粒体基因组保留了陆生植物线粒体基因组的原型特征。
The phylogenetic positions of bryophytes and charophytes, together with their genome features, are important for understanding early land plant evolution. Here we report the complete nucleotide sequence (105,340 bp) of the circular-mapping mitochondrial DNA of the moss Physcomitrella patens. Available evidence suggests that the multipartite structure of the mitochondrial genome in flowering plants does not occur in Physcomitrella. It contains genes for 3 rRNAs (rnl, rns, and rrn5), 24 tRNAs, and 42 conserved mitochondrial proteins (14 ribosomal proteins, 4 ccm proteins, 9 nicotinamide adenine dinucleotide dehydrogenase subunits, 5 ATPase subunits, 2 succinate dehydrogenase subunits, apocytochrome b, 3 cytochrome oxidase subunits, and 4 other proteins). We estimate that 5 tRNA genes are missing that might be encoded by the nuclear genome. The overall mitochondrial genome structure is similar in Physcomitrella, Chara vulgaris, Chaetosphaeridium globosum, and Marchantia polymorpha, with easily identifiable inversions and translocations. Significant synteny with angiosperm and chlorophyte mitochondrial genomes was not detected. Phylogenetic analysis of 18 conserved proteins suggests that the moss-liverwort clade is sister to angiosperms, which is consistent with a previous analysis of chloroplast genes but is not consistent with some analyses using mitochondrial sequences. In Physcomitrella, 27 introns are present within 16 genes. Nine of its intron positions are shared with angiosperms and 4 with Marchantia, which in turn shares only one intron position with angiosperms. The phylogenetic analysis as well as the syntenic structure suggest that the mitochondrial genomes of Physcomitrella and Marchantia retain prototype features among land plant mitochondrial genomes.