Robust Electrospun Nanofibers from Chemosynthetic Poly(4-hydroxybutyrate) as Artificial Dural Substitute.
Robust Electrospun Nanofibers from Chemosynthetic Poly(4-hydroxybutyrate) as Artificial Dural Substitute.
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DOI：
10.1002/mabi.202100134
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发表时间：
2021-05
影响因子：
4.6
通讯作者：
Huihui Ma;Yilin Sun;Ying Tang;Yong Shen;Ze Kan;Qing Li;Sha Fang;Yingxi Lu;Xianfeng Zhou;Zhibo Li
中科院分区：
文献类型：
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作者：
Huihui Ma;Yilin Sun;Ying Tang;Yong Shen;Ze Kan;Qing Li;Sha Fang;Yingxi Lu;Xianfeng Zhou;Zhibo Li
生物可吸收聚（4-羟基丁酸酯）（P4 HB）可以满足硬脑膜替代物所需的特定要求，包括其高弹性，长期强度保持性能和生物相容性，而不会产生大量的酸性降解产物。然而，商业化的P4 HB只能通过细菌发酵来生产，这限制了其在脑脊髓系统中的应用，这是由于较高的内毒素限制。同时，通过γ-丁内酯的开环聚合，可以得到P4 HB。在此贡献中，来自化学合成的高分子量P4 HB被电纺成纤维膜，显示出与天然硬脑膜相匹配的良好机械性能。这种P4 HB膜在体外诱导成纤维细胞的快速细胞迁移、粘附和增殖。大鼠皮下植入实验表明，P4 HB膜具有良好的生物相容性和良好的生物降解性能。在作为覆盖移植物植入兔硬脑膜缺损模型后，P4 HB膜可防止脑脊液漏并再生硬脑膜组织，而未检测到任何局部或全身感染或异物反应。因此，静电纺丝P4 HB膜可能特别有用的人工硬脑膜替代品，以诱导伤口闭合和组织再生，这将是非常有益的神经外科在未来。
Bioresorbable poly(4-hydroxybutyrate) (P4HB) may fulfill the specific requirements that are necessary for a dural substitute, including its high elasticity, long-term strength retention properties, and the biocompatibility without significant accumulation of acidic degradation products. However, commercial P4HB can only be produced by the bacterial fermentation, which limits its applications in the cerebrospinal system due to higher endotoxin restriction. Meanwhile, P4HB can be prepared via the ring-opening polymerization of γ-butyrolactone. In this contribution, high molecular weight P4HB from chemosynthesis is electrospun into fibrous membrane, showing good mechanical properties that match the natural dura mater. Such P4HB membrane induces fast cellular migration, adhesion, and proliferation of fibroblasts in vitro. Subcutaneous implantation in rats demonstrates excellent biocompatibility of the P4HB membrane with proper biodegradation behaviors. After implantation in the rabbit dural defect model as an onlay graft, the P4HB membranes prevent cerebrospinal fluid leakage and regenerate dura tissue without detecting any local or systematic infections or foreign body responses. Thus, the electrospun P4HB membranes may be particularly useful as artificial dural substitutes to induce wound closure and tissue regeneration, which will be of great benefit to neurosurgery in the future.